BRENDA - Enzyme Database

Characterization of phospholipase A from pulmonary secretions of patients with alveolar proteinosis

Sahu, S.; Lynn, W.S.; Biochim. Biophys. Acta 489, 307-317 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
deoxycholate
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
75000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Homo sapiens
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
ion-exchange chromatography, gel-filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
lung
pulmonary secretions
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
1140
-
substrate: egg yolk emulsion
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.4
-20°C, lyophilized enzyme, no loss of activity over 3 months
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
133918
Homo sapiens
1-myristoyl-phosphorylcholine + myristic acid
-
133918
Homo sapiens
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
133918
Homo sapiens
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
133918
Homo sapiens
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylethanolamine + H2O
-
133918
Homo sapiens
1-palmitoyl-phosphatidylethanolamine + palmitic acid
-
133918
Homo sapiens
?
3.1.1.4
1-oleoyl-2-palmitoyl-phosphatidylcholine + H2O
-
133918
Homo sapiens
1-oleoyl-phosphatidylcholine + palmitic acid
-
133918
Homo sapiens
?
3.1.1.4
1-palmitoyl-2-oleoylphosphatidylcholine + H2O
-
133918
Homo sapiens
1-palmitoyl-glycerophosphocholine + oleic acid
-
133918
Homo sapiens
?
3.1.1.4
diarachidonylphosphatidylcholine + H2O
-
133918
Homo sapiens
arachidonylphosphatidylcholine + arachidonic acid
-
133918
Homo sapiens
?
3.1.1.4
phosphatidylcholine + H2O
-
133918
Homo sapiens
1-acylglycerophosphocholine + fatty acid
-
133918
Homo sapiens
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133918
Homo sapiens
1-acylglycerophosphorylethanolamine + fatty acid
-
133918
Homo sapiens
?
3.1.1.4
phosphatidylglycerol + H2O
-
133918
Homo sapiens
1-acylglycerol + fatty acid
-
133918
Homo sapiens
?
3.1.1.4
phospholipids + H2O
-
133918
Homo sapiens
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
7.5
8.5
substrate: egg yolk emulsion
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
deoxycholate
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
75000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Homo sapiens
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
ion-exchange chromatography, gel-filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
lung
pulmonary secretions
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
1140
-
substrate: egg yolk emulsion
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.4
-20°C, lyophilized enzyme, no loss of activity over 3 months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
133918
Homo sapiens
1-myristoyl-phosphorylcholine + myristic acid
-
133918
Homo sapiens
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
133918
Homo sapiens
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
133918
Homo sapiens
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylethanolamine + H2O
-
133918
Homo sapiens
1-palmitoyl-phosphatidylethanolamine + palmitic acid
-
133918
Homo sapiens
?
3.1.1.4
1-oleoyl-2-palmitoyl-phosphatidylcholine + H2O
-
133918
Homo sapiens
1-oleoyl-phosphatidylcholine + palmitic acid
-
133918
Homo sapiens
?
3.1.1.4
1-palmitoyl-2-oleoylphosphatidylcholine + H2O
-
133918
Homo sapiens
1-palmitoyl-glycerophosphocholine + oleic acid
-
133918
Homo sapiens
?
3.1.1.4
diarachidonylphosphatidylcholine + H2O
-
133918
Homo sapiens
arachidonylphosphatidylcholine + arachidonic acid
-
133918
Homo sapiens
?
3.1.1.4
phosphatidylcholine + H2O
-
133918
Homo sapiens
1-acylglycerophosphocholine + fatty acid
-
133918
Homo sapiens
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133918
Homo sapiens
1-acylglycerophosphorylethanolamine + fatty acid
-
133918
Homo sapiens
?
3.1.1.4
phosphatidylglycerol + H2O
-
133918
Homo sapiens
1-acylglycerol + fatty acid
-
133918
Homo sapiens
?
3.1.1.4
phospholipids + H2O
-
133918
Homo sapiens
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
7.5
8.5
substrate: egg yolk emulsion
Homo sapiens