BRENDA - Enzyme Database

Properties of purified detergent-resistant phospholipase A of Escherichia coli K-12

Tamori, Y.; Nishijima, M.; Nojima, S.; J. Biochem. 86, 1129-1138 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
Triton X-100
-
Escherichia coli
General Stability
EC Number
General Stability
Organism
3.1.1.4
detergent-resistant enzyme, Triton X-100 prevents the enzymes thermal inactivation at 37øC
Escherichia coli
3.1.1.4
sodium cholate prevents the enzymes thermal inactivation at 37øC
Escherichia coli
3.1.1.4
sodium deoxycholate prevents the enzymes thermal inactivation at 37øC
Escherichia coli
3.1.1.4
Span 40 prevents the enzymes thermal inactivation at 37›C >
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.067
-
phosphatidylethanolamine
active enzyme
Escherichia coli
3.1.1.4
0.167
-
phosphatidylethanolamine
inactivated enzyme
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
plasma membrane
-
Escherichia coli
5886
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
21000
-
SDS-PAGE
Escherichia coli
3.1.1.4
29000
-
SDS-PAGE, enzyme previously inactivated by heat treatment at 80°C or boiled in solution containing SDS
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Escherichia coli
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Escherichia coli
-
K-12
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
gel chromatography
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.4
-20°C
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
-
133914
Escherichia coli
1-acylglycerophosphocholine + fatty acid
-
133914
Escherichia coli
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133914
Escherichia coli
1-acylglycerophosphorylethanolamine + fatty acid
-
133914
Escherichia coli
?
3.1.1.4
phospholipids + H2O
-
133914
Escherichia coli
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.4
37
43
-
Escherichia coli
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
30
70
aqueous solution of purified enzyme, with Triton X-100
Escherichia coli
3.1.1.4
37
70
aqueous solution of purified enzyme, without Triton X-100
Escherichia coli
3.1.1.4
100
-
crude preparation, even activation observable
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
Triton X-100
-
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.4
detergent-resistant enzyme, Triton X-100 prevents the enzymes thermal inactivation at 37øC
Escherichia coli
3.1.1.4
sodium cholate prevents the enzymes thermal inactivation at 37øC
Escherichia coli
3.1.1.4
sodium deoxycholate prevents the enzymes thermal inactivation at 37øC
Escherichia coli
3.1.1.4
Span 40 prevents the enzymes thermal inactivation at 37›C >
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.067
-
phosphatidylethanolamine
active enzyme
Escherichia coli
3.1.1.4
0.167
-
phosphatidylethanolamine
inactivated enzyme
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
plasma membrane
-
Escherichia coli
5886
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
21000
-
SDS-PAGE
Escherichia coli
3.1.1.4
29000
-
SDS-PAGE, enzyme previously inactivated by heat treatment at 80°C or boiled in solution containing SDS
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Escherichia coli
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
gel chromatography
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.4
-20°C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
-
133914
Escherichia coli
1-acylglycerophosphocholine + fatty acid
-
133914
Escherichia coli
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133914
Escherichia coli
1-acylglycerophosphorylethanolamine + fatty acid
-
133914
Escherichia coli
?
3.1.1.4
phospholipids + H2O
-
133914
Escherichia coli
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.4
37
43
-
Escherichia coli
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
30
70
aqueous solution of purified enzyme, with Triton X-100
Escherichia coli
3.1.1.4
37
70
aqueous solution of purified enzyme, without Triton X-100
Escherichia coli
3.1.1.4
100
-
crude preparation, even activation observable
Escherichia coli