EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.4 | Triton X-100 | - |
Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.4 | detergent-resistant enzyme, Triton X-100 prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
3.1.1.4 | sodium cholate prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
3.1.1.4 | sodium deoxycholate prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
3.1.1.4 | Span 40 prevents the enzymes thermal inactivation at 37C > | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.4 | 0.067 | - |
phosphatidylethanolamine | active enzyme | Escherichia coli | |
3.1.1.4 | 0.167 | - |
phosphatidylethanolamine | inactivated enzyme | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.4 | plasma membrane | - |
Escherichia coli | 5886 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 21000 | - |
SDS-PAGE | Escherichia coli |
3.1.1.4 | 29000 | - |
SDS-PAGE, enzyme previously inactivated by heat treatment at 80°C or boiled in solution containing SDS | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | phospholipids + H2O | Escherichia coli | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.4 | Escherichia coli | - |
K-12 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.4 | gel chromatography | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.4 | -20°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | phosphatidylcholine + H2O | - |
Escherichia coli | 1-acylglycerophosphocholine + fatty acid | - |
? | |
3.1.1.4 | phosphatidylethanolamine + H2O | - |
Escherichia coli | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
3.1.1.4 | phospholipids + H2O | - |
Escherichia coli | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 37 | 43 | - |
Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 30 | 70 | aqueous solution of purified enzyme, with Triton X-100 | Escherichia coli |
3.1.1.4 | 37 | 70 | aqueous solution of purified enzyme, without Triton X-100 | Escherichia coli |
3.1.1.4 | 100 | - |
crude preparation, even activation observable | Escherichia coli |