BRENDA - Enzyme Database

Mode of action of a bacteriocin from Erwinia carotovora III. Properties of phospholipase A of Erwinia carotovora and its involvement in phospholipid degradation caused by carotovoricin

Itoh, Y.; Iwata, T.; Izaki, K.; Takahashi, H.; J. Gen. Appl. Microbiol. 27, 239-251 (1981)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
cholate
-
Pectobacterium carotovorum
3.1.1.4
deoxycholate
-
Pectobacterium carotovorum
3.1.1.4
methanol
-
Pectobacterium carotovorum
3.1.1.4
Triton X-100
-
Pectobacterium carotovorum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
EDTA
-
Pectobacterium carotovorum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
plasma membrane
-
Pectobacterium carotovorum
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Pectobacterium carotovorum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Pectobacterium carotovorum
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Pectobacterium carotovorum
-
bacteria
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.4
0.0379
-
substrate: phosphatidylethanolamine
Pectobacterium carotovorum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
phosphatidylethanolamine + H2O
-
133909
Pectobacterium carotovorum
1-acylglycerophosphorylethanolamine + fatty acid
-
133909
Pectobacterium carotovorum
?
3.1.1.4
phosphatidylglycerol + H2O
-
133909
Pectobacterium carotovorum
1-acylglycerol + fatty acid
-
133909
Pectobacterium carotovorum
?
3.1.1.4
phospholipids + H2O
-
133909
Pectobacterium carotovorum
?
-
-
-
?
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.1.1.4
40
80
for 10 min., slight activation
Pectobacterium carotovorum
3.1.1.4
100
-
for 10 min, only 8% loss in activity
Pectobacterium carotovorum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
7.2
-
substrate: phosphatidylethanolamine
Pectobacterium carotovorum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
cholate
-
Pectobacterium carotovorum
3.1.1.4
deoxycholate
-
Pectobacterium carotovorum
3.1.1.4
methanol
-
Pectobacterium carotovorum
3.1.1.4
Triton X-100
-
Pectobacterium carotovorum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
EDTA
-
Pectobacterium carotovorum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
plasma membrane
-
Pectobacterium carotovorum
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Pectobacterium carotovorum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Pectobacterium carotovorum
-
?
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.4
0.0379
-
substrate: phosphatidylethanolamine
Pectobacterium carotovorum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
phosphatidylethanolamine + H2O
-
133909
Pectobacterium carotovorum
1-acylglycerophosphorylethanolamine + fatty acid
-
133909
Pectobacterium carotovorum
?
3.1.1.4
phosphatidylglycerol + H2O
-
133909
Pectobacterium carotovorum
1-acylglycerol + fatty acid
-
133909
Pectobacterium carotovorum
?
3.1.1.4
phospholipids + H2O
-
133909
Pectobacterium carotovorum
?
-
-
-
?
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.1.1.4
40
80
for 10 min., slight activation
Pectobacterium carotovorum
3.1.1.4
100
-
for 10 min, only 8% loss in activity
Pectobacterium carotovorum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
7.2
-
substrate: phosphatidylethanolamine
Pectobacterium carotovorum