BRENDA - Enzyme Database

Isolation of phospholipase A2 from red cell membranes of sheep

Zahler, P.; Kramer, R.; Methods Enzymol. 71, 690-698 (1981)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.1.4
cholate restores full activity of SDS-treated PLA2
Ovis aries
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
plasma membrane
-
Ovis aries
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Ovis aries
3.1.1.4
Mg2+
can replace Ca2+, decreased activity
Ovis aries
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
12000
-
gel filtration
Ovis aries
3.1.1.4
18500
-
SDS-PAGE
Ovis aries
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
Ovis aries
-
diacylglycerol + phosphorylcholine
-
-
?
3.1.1.4
phospholipids + H2O
Ovis aries
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Ovis aries
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
gel filtration, affinity chromatography, 2835fold
Ovis aries
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
erythrocyte
-
Ovis aries
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.4
4.725
-
substrate: phosphatidylcholine
Ovis aries
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
-
133908
Ovis aries
1-acylglycerophosphocholine + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phosphatidylcholine + H2O
-
133908
Ovis aries
diacylglycerol + phosphorylcholine
-
-
-
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133908
Ovis aries
1-acylglycerophosphorylethanolamine + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phosphatidylglycerol + H2O
-
133908
Ovis aries
1-acylglycerol + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phosphatidylserine + H2O
-
133908
Ovis aries
1-acylglycerophosphoserine + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phospholipids + H2O
-
133908
Ovis aries
?
-
-
-
?
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.4
cholate restores full activity of SDS-treated PLA2
Ovis aries
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
plasma membrane
-
Ovis aries
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Ovis aries
3.1.1.4
Mg2+
can replace Ca2+, decreased activity
Ovis aries
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
12000
-
gel filtration
Ovis aries
3.1.1.4
18500
-
SDS-PAGE
Ovis aries
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
Ovis aries
-
diacylglycerol + phosphorylcholine
-
-
?
3.1.1.4
phospholipids + H2O
Ovis aries
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
gel filtration, affinity chromatography, 2835fold
Ovis aries
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
erythrocyte
-
Ovis aries
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.4
4.725
-
substrate: phosphatidylcholine
Ovis aries
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
-
133908
Ovis aries
1-acylglycerophosphocholine + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phosphatidylcholine + H2O
-
133908
Ovis aries
diacylglycerol + phosphorylcholine
-
-
-
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133908
Ovis aries
1-acylglycerophosphorylethanolamine + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phosphatidylglycerol + H2O
-
133908
Ovis aries
1-acylglycerol + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phosphatidylserine + H2O
-
133908
Ovis aries
1-acylglycerophosphoserine + fatty acid
-
133908
Ovis aries
?
3.1.1.4
phospholipids + H2O
-
133908
Ovis aries
?
-
-
-
?