BRENDA - Enzyme Database

Phospholipase A2 from cobra venom (Naja naja naja)

Deems, R.A.; Dennis, E.A.; Methods Enzymol. 71, 703-710 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
phosphatidylcholine
activates hydrolysis of phosphatidylethanolamine
Naja naja
3.1.1.4
sphingomyelin
activates hydrolysis of phosphatidylethanolamine
Naja naja
General Stability
EC Number
General Stability
Organism
3.1.1.4
guanidine-HCL, PLA2 retains 60% of activity when assay contains 6 M of guanidine-HCl
Naja naja
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Ba2+
-
Naja naja
3.1.1.4
p-bromophenacyl bromide
-
Naja naja
3.1.1.4
Sr2+
competitive inhibition
Naja naja
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
2
5
phosphatidylcholine
Triton X-100/phospholipid: 2/1
Naja naja
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Naja naja
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
13000
-
gel filtration, SDS-PAGE, analytical ultracentrifugation
Naja naja
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
Naja naja
-
diacylglycerol + phosphorylcholine
-
-
?
3.1.1.4
phospholipids + H2O
Naja naja
-
?
-
-
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.1.4
Acetone
PLA2 regains almost full activity when added to assay mixture
Naja naja
3.1.1.4
benzene
PLA2 regains almost full activity when added to assay mixture
Naja naja
3.1.1.4
chloroform
PLA2 regains almost full activity when added to assay mixture
Naja naja
3.1.1.4
ether
PLA2 regains almost full activity when added to assay mixture
Naja naja
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Naja naja
-
cobra
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
column chromatography, 15fold
Naja naja
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
1129
-
substrate: 1,2-diacyl-phosphatidylcholine
Naja naja
3.1.1.4
2000
-
Triton X-100/phospholipid: 2/1
Naja naja
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.4
frozen, PLA2 concentration below 2 mg/ml, no loss of activity or precipitation for up to 12 months
Naja naja
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphorylcholine + H2O
-
133906
Naja naja
1-acyl-sn-glycero-3-phosphorylcholine + fatty acid
-
133906
Naja naja
?
3.1.1.4
phosphatidylcholine + H2O
-
133906
Naja naja
diacylglycerol + phosphorylcholine
-
-
-
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133906
Naja naja
1-acylglycerophosphorylethanolamine + fatty acid
-
133906
Naja naja
?
3.1.1.4
phosphatidylserine + H2O
-
133906
Naja naja
1-acylglycerophosphoserine + fatty acid
-
133906
Naja naja
?
3.1.1.4
phospholipids + H2O
-
133906
Naja naja
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.4
dimer
2 * 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, dimerization occurs at enzyme concentrations between 0.1 and 2 mg PLA2 per ml
Naja naja
3.1.1.4
monomer
monomeric when enzyme concentration is below 0.05 mg PLA2 per ml, 13000, gel filtration, SDS-PAGE, analytical ultracentifugation
Naja naja
3.1.1.4
polymer
13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, polimerization at enzyme concentrations above 5mg PLA2 per ml
Naja naja
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
100
-
for 10 min at pH 3-4, only 5% loss in activity
Naja naja
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.4
7
9
substrate: 1,2-diacyl-phosphatidylcholine
Naja naja
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
phosphatidylcholine
activates hydrolysis of phosphatidylethanolamine
Naja naja
3.1.1.4
sphingomyelin
activates hydrolysis of phosphatidylethanolamine
Naja naja
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.4
guanidine-HCL, PLA2 retains 60% of activity when assay contains 6 M of guanidine-HCl
Naja naja
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Ba2+
-
Naja naja
3.1.1.4
p-bromophenacyl bromide
-
Naja naja
3.1.1.4
Sr2+
competitive inhibition
Naja naja
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
2
5
phosphatidylcholine
Triton X-100/phospholipid: 2/1
Naja naja
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Naja naja
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
13000
-
gel filtration, SDS-PAGE, analytical ultracentrifugation
Naja naja
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phosphatidylcholine + H2O
Naja naja
-
diacylglycerol + phosphorylcholine
-
-
?
3.1.1.4
phospholipids + H2O
Naja naja
-
?
-
-
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.1.4
Acetone
PLA2 regains almost full activity when added to assay mixture
Naja naja
3.1.1.4
benzene
PLA2 regains almost full activity when added to assay mixture
Naja naja
3.1.1.4
chloroform
PLA2 regains almost full activity when added to assay mixture
Naja naja
3.1.1.4
ether
PLA2 regains almost full activity when added to assay mixture
Naja naja
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
column chromatography, 15fold
Naja naja
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
1129
-
substrate: 1,2-diacyl-phosphatidylcholine
Naja naja
3.1.1.4
2000
-
Triton X-100/phospholipid: 2/1
Naja naja
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.4
frozen, PLA2 concentration below 2 mg/ml, no loss of activity or precipitation for up to 12 months
Naja naja
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphorylcholine + H2O
-
133906
Naja naja
1-acyl-sn-glycero-3-phosphorylcholine + fatty acid
-
133906
Naja naja
?
3.1.1.4
phosphatidylcholine + H2O
-
133906
Naja naja
diacylglycerol + phosphorylcholine
-
-
-
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133906
Naja naja
1-acylglycerophosphorylethanolamine + fatty acid
-
133906
Naja naja
?
3.1.1.4
phosphatidylserine + H2O
-
133906
Naja naja
1-acylglycerophosphoserine + fatty acid
-
133906
Naja naja
?
3.1.1.4
phospholipids + H2O
-
133906
Naja naja
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.4
dimer
2 * 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, dimerization occurs at enzyme concentrations between 0.1 and 2 mg PLA2 per ml
Naja naja
3.1.1.4
monomer
monomeric when enzyme concentration is below 0.05 mg PLA2 per ml, 13000, gel filtration, SDS-PAGE, analytical ultracentifugation
Naja naja
3.1.1.4
polymer
13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, polimerization at enzyme concentrations above 5mg PLA2 per ml
Naja naja
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
100
-
for 10 min at pH 3-4, only 5% loss in activity
Naja naja
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.4
7
9
substrate: 1,2-diacyl-phosphatidylcholine
Naja naja