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Literature summary extracted from

  • De Geus, P.; van den Bergh, C.J.; Kuipers, O.; Verheij, H.M.; Hockstra, W.P.M.; de Haas, G.H.
    Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli (1987), Nucleic Acids Res., 15, 3743-3759.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 into pBR322 and pCI1857, transformation of Escherichia coli strains HB101 and AB1157 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 14000
-
 gel filtration, amino acid composition Sus scrofa
3.1.1.4 15000
-
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Sus scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Sus scrofa P00592
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 on CM ion exchange resin, over 90% pure Sus scrofa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4 S-sulfonation method Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 10
-
 substrate: monomeric dihexanoyl-dithio-phosphatidylcholine, proPLA2 Sus scrofa
3.1.1.4 2000
-
 substrate: micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, active PLA2 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O micellar substrate Sus scrofa 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 dihexanoyl-dithio-phosphatidylcholine + H2O proPLA2, monomeric substrate Sus scrofa ?
-
 ?
3.1.1.4 phospholipids + H2O
-
 Sus scrofa ?
-
 ?