BRENDA - Enzyme Database

The purification and characterization of a phospholipase A in hamster heart cytosol for the hydrolysis of phosphatidylcholine

Cao, Y.Z.; Tam, S.W.; Arthur, G.; Chen, H.; Choy, P.C.; J. Biol. Chem. 262, 16927-16935 (1987)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
anti-peptide antibody
-
Mesocricetus auratus
3.1.1.4
Sodium dodecyl sulfate
-
Mesocricetus auratus
3.1.1.4
Triton X-100
-
Mesocricetus auratus
3.1.1.4
Tween 20
-
Mesocricetus auratus
3.1.1.32
rabbit anti-phospholipase A antibodies
-
Mesocricetus auratus
3.1.1.32
SDS
-
Mesocricetus auratus
3.1.1.32
Triton X-100
-
Mesocricetus auratus
3.1.1.32
Tween 20
-
Mesocricetus auratus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.5
-
1-palmitoyl-2-arachidonoylphosphatidylcholine
-
Mesocricetus auratus
3.1.1.4
0.5
-
1-stearyl-2-arachidonoylphosphatidylcholine
-
Mesocricetus auratus
3.1.1.32
0.5
-
1-palmitoyl-2-arachidonylglycerophosphocholine
-
Mesocricetus auratus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
cytoplasm
-
Mesocricetus auratus
5737
-
3.1.1.32
cytosol
-
Mesocricetus auratus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
Ca2+
10 mM, 10% activation
Mesocricetus auratus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
14000
-
SDS-PAGE, Mr of one subunit
Mesocricetus auratus
3.1.1.4
140000
-
gel filtration, multimeric complex
Mesocricetus auratus
3.1.1.32
14000
-
x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE
Mesocricetus auratus
3.1.1.32
140000
-
gel filtration
Mesocricetus auratus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Mesocricetus auratus
cardiac contraction and excitation
?
-
-
?
3.1.1.32
additional information
Mesocricetus auratus
the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Mesocricetus auratus
-
-
-
3.1.1.32
Mesocricetus auratus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
ion-exchange chromatography, gel-filtration chromatography,167fold
Mesocricetus auratus
3.1.1.32
-
Mesocricetus auratus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
heart
-
Mesocricetus auratus
-
3.1.1.32
heart
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
0.0192
-
substrate: phosphatidylcholine
Mesocricetus auratus
3.1.1.32
1.155
-
-
Mesocricetus auratus
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.4
-20°C, 50 mM Hepes, 5 mM EDTA, 48 hours without loss of activity, 75% of the initial activity remains after one week, storage at -70°C does not improve the enzymes stability
Mesocricetus auratus
3.1.1.32
-20°C, 50 mM Hepes, 5 mM EDTA, pH 7.4, stable for 48 h, 25% loss of activity after 1 week
Mesocricetus auratus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
133898
Mesocricetus auratus
?
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-palmitoyl-glycerophosphorylcholine + linolic acid
-
133898
Mesocricetus auratus
?
3.1.1.4
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-stearoyl-glycerophosphorylcholine + arachidonic acid
-
133898
Mesocricetus auratus
?
3.1.1.4
phosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-acylglycerophosphocholine + fatty acid
-
133898
Mesocricetus auratus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133898
Mesocricetus auratus
1-acylglycerophosphorylethanolamine + fatty acid
-
133898
Mesocricetus auratus
?
3.1.1.4
phospholipids + H2O
cardiac contraction and excitation
133898
Mesocricetus auratus
?
-
-
-
?
3.1.1.32
1-palmitoyl-2-arachidonoylglycerophosphocholine + H2O
-
133898
Mesocricetus auratus
palmitic acid + 2-arachidonoylglycerophosphocholine
-
-
-
?
3.1.1.32
1-stearoyl-2-arachidonoylglycerophosphocholine + H2O
-
133898
Mesocricetus auratus
stearic acid + 2-arachidonoylglycerophosphocholine
-
-
-
?
3.1.1.32
1-stearoyl-2-arachidonoylglycerophosphoinositol + H2O
-
133898
Mesocricetus auratus
stearic acid + 2-arachidonoylglycerophosphoinositol
-
-
-
?
3.1.1.32
additional information
the enzyme exhibits both phospholipase A1 and phospholipase A2 activity
133898
Mesocricetus auratus
?
-
-
-
-
3.1.1.32
additional information
the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids
133898
Mesocricetus auratus
?
-
-
-
-
3.1.1.32
phosphatidylcholine + H2O
-
133898
Mesocricetus auratus
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
133898
Mesocricetus auratus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.4
hexamer
6 * 14000, identical subunits, at least six subunits could be cross-linked
Mesocricetus auratus
3.1.1.32
?
x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE
Mesocricetus auratus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
9
-
substrate: 1-palmitoyl-2-arachidonoylphosphatidylcholine
Mesocricetus auratus
3.1.1.32
9
-
-
Mesocricetus auratus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
anti-peptide antibody
-
Mesocricetus auratus
3.1.1.4
Sodium dodecyl sulfate
-
Mesocricetus auratus
3.1.1.4
Triton X-100
-
Mesocricetus auratus
3.1.1.4
Tween 20
-
Mesocricetus auratus
3.1.1.32
rabbit anti-phospholipase A antibodies
-
Mesocricetus auratus
3.1.1.32
SDS
-
Mesocricetus auratus
3.1.1.32
Triton X-100
-
Mesocricetus auratus
3.1.1.32
Tween 20
-
Mesocricetus auratus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.5
-
1-palmitoyl-2-arachidonoylphosphatidylcholine
-
Mesocricetus auratus
3.1.1.4
0.5
-
1-stearyl-2-arachidonoylphosphatidylcholine
-
Mesocricetus auratus
3.1.1.32
0.5
-
1-palmitoyl-2-arachidonylglycerophosphocholine
-
Mesocricetus auratus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
cytoplasm
-
Mesocricetus auratus
5737
-
3.1.1.32
cytosol
-
Mesocricetus auratus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
Ca2+
10 mM, 10% activation
Mesocricetus auratus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
14000
-
SDS-PAGE, Mr of one subunit
Mesocricetus auratus
3.1.1.4
140000
-
gel filtration, multimeric complex
Mesocricetus auratus
3.1.1.32
14000
-
x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE
Mesocricetus auratus
3.1.1.32
140000
-
gel filtration
Mesocricetus auratus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Mesocricetus auratus
cardiac contraction and excitation
?
-
-
?
3.1.1.32
additional information
Mesocricetus auratus
the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
ion-exchange chromatography, gel-filtration chromatography,167fold
Mesocricetus auratus
3.1.1.32
-
Mesocricetus auratus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
heart
-
Mesocricetus auratus
-
3.1.1.32
heart
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
0.0192
-
substrate: phosphatidylcholine
Mesocricetus auratus
3.1.1.32
1.155
-
-
Mesocricetus auratus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.4
-20°C, 50 mM Hepes, 5 mM EDTA, 48 hours without loss of activity, 75% of the initial activity remains after one week, storage at -70°C does not improve the enzymes stability
Mesocricetus auratus
3.1.1.32
-20°C, 50 mM Hepes, 5 mM EDTA, pH 7.4, stable for 48 h, 25% loss of activity after 1 week
Mesocricetus auratus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
133898
Mesocricetus auratus
?
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-palmitoyl-glycerophosphorylcholine + linolic acid
-
133898
Mesocricetus auratus
?
3.1.1.4
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-stearoyl-glycerophosphorylcholine + arachidonic acid
-
133898
Mesocricetus auratus
?
3.1.1.4
phosphatidylcholine + H2O
-
133898
Mesocricetus auratus
1-acylglycerophosphocholine + fatty acid
-
133898
Mesocricetus auratus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133898
Mesocricetus auratus
1-acylglycerophosphorylethanolamine + fatty acid
-
133898
Mesocricetus auratus
?
3.1.1.4
phospholipids + H2O
cardiac contraction and excitation
133898
Mesocricetus auratus
?
-
-
-
?
3.1.1.32
1-palmitoyl-2-arachidonoylglycerophosphocholine + H2O
-
133898
Mesocricetus auratus
palmitic acid + 2-arachidonoylglycerophosphocholine
-
-
-
?
3.1.1.32
1-stearoyl-2-arachidonoylglycerophosphocholine + H2O
-
133898
Mesocricetus auratus
stearic acid + 2-arachidonoylglycerophosphocholine
-
-
-
?
3.1.1.32
1-stearoyl-2-arachidonoylglycerophosphoinositol + H2O
-
133898
Mesocricetus auratus
stearic acid + 2-arachidonoylglycerophosphoinositol
-
-
-
?
3.1.1.32
additional information
the enzyme exhibits both phospholipase A1 and phospholipase A2 activity
133898
Mesocricetus auratus
?
-
-
-
-
3.1.1.32
additional information
the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids
133898
Mesocricetus auratus
?
-
-
-
-
3.1.1.32
phosphatidylcholine + H2O
-
133898
Mesocricetus auratus
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
133898
Mesocricetus auratus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.4
hexamer
6 * 14000, identical subunits, at least six subunits could be cross-linked
Mesocricetus auratus
3.1.1.32
?
x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE
Mesocricetus auratus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
9
-
substrate: 1-palmitoyl-2-arachidonoylphosphatidylcholine
Mesocricetus auratus
3.1.1.32
9
-
-
Mesocricetus auratus