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Literature summary extracted from

  • Filgueiras, O.M.O.; Possmayer, F.
    Purification and characterization of a phospholipase A2 associated with rabbit lung microsomes: some evidence for its mitochondrial origin (1990), Biochim. Biophys. Acta, 1046, 258-266.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 2-mercaptoethanol
-
 Oryctolagus cuniculus
3.1.1.4 EDTA
-
 Oryctolagus cuniculus
3.1.1.4 iodoacetamide weak inhibitor Oryctolagus cuniculus
3.1.1.4 N-ethylmaleimide weak inhibitor Oryctolagus cuniculus
3.1.1.4 p-bromophenacyl bromide
-
 Oryctolagus cuniculus
3.1.1.4 p-hydroxymercuribenzoate weak inhibitor Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.027
-
 1-palmitoyl-2-arachidonoylphosphatidylethanolamine at pH 8.5, without detergents Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 lysosome
-
 Oryctolagus cuniculus 5764
-
3.1.1.4 microsome
-
 Oryctolagus cuniculus
-
-
3.1.1.4 mitochondrion
-
 Oryctolagus cuniculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Oryctolagus cuniculus
3.1.1.4 Cd2+ 1% of the activity obtained with Ca2+ Oryctolagus cuniculus
3.1.1.4 Sr2+ 1% of the activity obtained with Ca2+ Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 6500
-
 gel filtration, underestimation seems obvious to authors Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Oryctolagus cuniculus inflammatory diseases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 ion-exchange chromatography, gel-filtration chromatography, hydroxyapatite chromatography, 72500fold Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 lung
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 290
-
 substrate: 1-palmitoyl-2-oleolylphosphatidylcholine Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 -20°C, in buffer A, 71 days, 4% decrease of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Oryctolagus cuniculus 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonoylphosphatidylethanolamine
-
 Oryctolagus cuniculus 1-palmitoyl-glycerophosphorylethanolamine + arachidonic acid
-
 ?
3.1.1.4 1-palmitoyl-2-oleoylphosphatidylcholine + H2O
-
 Oryctolagus cuniculus 1-palmitoyl-glycerophosphocholine + oleic acid
-
 ?
3.1.1.4 phospholipids + H2O inflammatory diseases Oryctolagus cuniculus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 8
-
 substrate: phosphatidylcholine Oryctolagus cuniculus
3.1.1.4 9.5 10 substrate: phosphatidylethanolamine Oryctolagus cuniculus