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Literature summary extracted from

  • Franken, P.A.; van den Berg, L.; Huang, J.; Gunyuzlu, P.; Lugtigheid, R.B.; Verheij, H.M.; de Haas, G.H.
    Purification and characterization of a mutant human platelet phospholipase A2 expressed in Escherichia coli (1992), Eur. J. Biochem., 203, 89-98.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 into M13-vector mp8 for site directed mutagenesis, into pUEX 1 vector for tranformation of Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.4 M20L according the gapped-duplex method, using amber selection Homo sapiens
3.1.1.4 M8L according the gapped-duplex method, using amber selection Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 2-tetradecanoylaminohexanol-1-phosphocholine competitive inhibition, R-enantiomer is a more potent inhibitor Homo sapiens
3.1.1.4 2-tetradecanoylaminohexanol-1-phosphoethanolamine competitive inhibition, R-enantiomer is a more potent inhibitor Homo sapiens
3.1.1.4 2-tetradecanoylaminohexanol-1-phosphoglycol competitive inhibition, R-enantiomer is a more potent inhibitor Homo sapiens
3.1.1.4 EDTA
-
 Homo sapiens
3.1.1.4 EDTA
-
 Sus scrofa
3.1.1.4 p-bromophenacyl bromide
-
 Homo sapiens
3.1.1.4 p-bromophenacyl bromide
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.1
-
 1,2-dioctanoyl-sn-glycero-3-phosphoglycol
-
 Homo sapiens
3.1.1.4 0.1
-
 1,2-dioctanoyl-sn-glycero-3-phosphoglycol
-
 Sus scrofa
3.1.1.4 0.2
-
 1,2-dioctanoyl-sn-glycero-3-phosphocholine
-
 Homo sapiens
3.1.1.4 0.9
-
 1,2-dinonanoyl-sn-glycero-3-phosphocholine
-
 Homo sapiens
3.1.1.4 1.4
-
 1,2-dihexanoyl-sn-glycero-3-phosphocholine
-
 Sus scrofa
3.1.1.4 2.4
-
 1,2-dihexanoyl-sn-glycero-3-phosphocholine [M8L and M20L]-loop-deleted PLA2 Homo sapiens
3.1.1.4 2.8
-
 1,2-dihexanoyl-sn-glycero-3-phosphocholine loop deleted PLA2 Homo sapiens
3.1.1.4 3
-
 1,2-diheptanoyl-sn-glycero-3-phosphocholine
-
 Sus scrofa
3.1.1.4 3.2
-
 1,2-dioctanoyl-sn-glycero-3-phosphocholine
-
 Sus scrofa
3.1.1.4 3.6
-
 1,2-diheptanoyl-sn-glycero-3-phosphocholine
-
 Homo sapiens
3.1.1.4 4.3
-
 1,2-dihexanoyl-sn-glycero-3-phosphocholine
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Homo sapiens
3.1.1.4 Ca2+
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 14000
-
-
 Sus scrofa
3.1.1.4 14000
-
 heparin-sepharose affinity chromatography, SDS-PAGE after digestion with thrombin, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Homo sapiens influence on platelet aggregation ?
-
 ?
3.1.1.4 phospholipids + H2O Homo sapiens inflammatory diseases ?
-
 ?
3.1.1.4 phospholipids + H2O Homo sapiens release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins ?
-
 ?
3.1.1.4 phospholipids + H2O Sus scrofa release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Homo sapiens
-
-
-
3.1.1.4 Naja melanoleuca
-
-
-
3.1.1.4 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 ion exchange chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 pancreas
-
 Homo sapiens
-
3.1.1.4 pancreas
-
 Sus scrofa
-
3.1.1.4 platelet
-
 Homo sapiens
-
3.1.1.4 venom
-
 Naja melanoleuca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 25
-
 substrate: dioleolyl-sn-phosphatidylcholine Homo sapiens
3.1.1.4 29
-
 substrate: 1,2-dipalmitoyl-phosphatidylcholine Sus scrofa
3.1.1.4 30
-
 substrate: 1,2-dipalmitoyl-phosphatidylcholine Homo sapiens
3.1.1.4 57
-
 substrate: dimyristoyl-sn-phosphatidylcholine Homo sapiens
3.1.1.4 68
-
 substrate: dimyristoyl-sn-phosphatidylcholine Sus scrofa
3.1.1.4 80
-
 substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine Sus scrofa
3.1.1.4 81
-
 substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine Sus scrofa
3.1.1.4 118
-
 substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine Homo sapiens
3.1.1.4 124
-
 substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine Homo sapiens
3.1.1.4 125
-
 substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine Sus scrofa
3.1.1.4 155
-
 substrate: dioleolyl-sn-phosphatidylethanolamine Homo sapiens
3.1.1.4 162
-
 substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine Sus scrofa
3.1.1.4 171
-
 substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine Homo sapiens
3.1.1.4 172
-
 substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine Homo sapiens
3.1.1.4 208
-
 substrate: dioleolyl phosphatidylglycerol Homo sapiens
3.1.1.4 210
-
 substrate: dioleolyl-sn-phosphatidylethanolamine Naja melanoleuca
3.1.1.4 295
-
 substrate: dioleolyl-sn-phosphatidylethanolamine Sus scrofa
3.1.1.4 320
-
 substrate: dioleolyl-sn-phosphatidylcholine Sus scrofa
3.1.1.4 410
-
 substrate: dioleolyl-sn-phosphatidylcholine Naja melanoleuca
3.1.1.4 530
-
 substrate: dioleolyl phosphatidylglycerol Naja melanoleuca
3.1.1.4 615
-
 substrate: dioleolyl phosphatidylglycerol Sus scrofa
3.1.1.4 750
-
 substrate: dimyristoyl-sn-phosphatidylcholine Naja melanoleuca
3.1.1.4 1220
-
 substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Naja melanoleuca
3.1.1.4 3235
-
 substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine Naja melanoleuca
3.1.1.4 4650
-
 substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine Naja melanoleuca
3.1.1.4 8870
-
 substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine Naja melanoleuca
3.1.1.4 9300
-
 substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Homo sapiens
3.1.1.4 11400
-
 substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine Naja melanoleuca
3.1.1.4 16000
-
 substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 room temperature, in presence or absence of Ca2+, pH ranging from 4.5 to 9.0, after 7 days more than 95% of the activity remains Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
 ?
3.1.1.4 1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O
-
 Sus scrofa 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
 ?
3.1.1.4 1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O
-
 Naja melanoleuca 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
 ?
3.1.1.4 1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
 ?
3.1.1.4 1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
-
 Sus scrofa 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
 ?
3.1.1.4 1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
-
 Naja melanoleuca 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
 ?
3.1.1.4 1,2-diheptanoyl-sn-glycero-3-phophorylcholine + H2O
-
 Homo sapiens 1-heptanoyl-sn-glycero-3-phophorylcholine + heptanoate
-
 ?
3.1.1.4 1,2-diheptanoyl-sn-glycero-3-phophorylcholine + H2O
-
 Sus scrofa 1-heptanoyl-sn-glycero-3-phophorylcholine + heptanoate
-
 ?
3.1.1.4 1,2-diheptanoyl-sn-glycero-3-phophorylcholine + H2O
-
 Naja melanoleuca 1-heptanoyl-sn-glycero-3-phophorylcholine + heptanoate
-
 ?
3.1.1.4 1,2-diheptanoyl-sn-glycero-3-sulfate + H2O
-
 Homo sapiens 1-heptanoyl-sn-glycero-3-sulfate + heptanoate
-
 ?
3.1.1.4 1,2-diheptanoyl-sn-glycero-3-sulfate + H2O
-
 Sus scrofa 1-heptanoyl-sn-glycero-3-sulfate + heptanoate
-
 ?
3.1.1.4 1,2-diheptanoyl-sn-glycero-3-sulfate + H2O
-
 Naja melanoleuca 1-heptanoyl-sn-glycero-3-sulfate + heptanoate
-
 ?
3.1.1.4 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
 ?
3.1.1.4 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
 Sus scrofa 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
 ?
3.1.1.4 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
 Naja melanoleuca 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
 ?
3.1.1.4 1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
 ?
3.1.1.4 1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
 Sus scrofa 1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
 ?
3.1.1.4 1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
 Naja melanoleuca 1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
 ?
3.1.1.4 1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
 Homo sapiens 1-myristoyl-phosphorylcholine + myristic acid
-
 ?
3.1.1.4 1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
 Sus scrofa 1-myristoyl-phosphorylcholine + myristic acid
-
 ?
3.1.1.4 1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
 Naja melanoleuca 1-myristoyl-phosphorylcholine + myristic acid
-
 ?
3.1.1.4 1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-nonanoyl-sn-glycero-3-phosphocholine + nonanoate
-
 ?
3.1.1.4 1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
 Sus scrofa 1-nonanoyl-sn-glycero-3-phosphocholine + nonanoate
-
 ?
3.1.1.4 1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
 Naja melanoleuca 1-nonanoyl-sn-glycero-3-phosphocholine + nonanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Sus scrofa 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Naja melanoleuca 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
 Homo sapiens 1-octanoyl-sn-glycero-3-phosphoglycol + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
 Sus scrofa 1-octanoyl-sn-glycero-3-phosphoglycol + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
 Naja melanoleuca 1-octanoyl-sn-glycero-3-phosphoglycol + octanoate
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
 Homo sapiens 1-oleoyl-sn-phosphatidylcholine + oleate
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
 Sus scrofa 1-oleoyl-sn-phosphatidylcholine + oleate
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
 Naja melanoleuca 1-oleoyl-sn-phosphatidylcholine + oleate
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
 Homo sapiens 1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
 Sus scrofa 1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
 Naja melanoleuca 1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
 ?
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Homo sapiens 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Sus scrofa 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Naja melanoleuca 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 1-acyl-2-linoleoyl-3-phosphoethanolamine + H2O
-
 Homo sapiens 1-acyl-3-phosphoethanolamine + linolic acid
-
 ?
3.1.1.4 1-acyl-2-linoleoyl-3-phosphoethanolamine + H2O
-
 Sus scrofa 1-acyl-3-phosphoethanolamine + linolic acid
-
 ?
3.1.1.4 1-acyl-2-linoleoyl-3-phosphoethanolamine + H2O
-
 Naja melanoleuca 1-acyl-3-phosphoethanolamine + linolic acid
-
 ?
3.1.1.4 dioleoyl phosphatidylglycerol + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.4 dioleoyl phosphatidylglycerol + H2O
-
 Sus scrofa ?
-
 ?
3.1.1.4 dioleoyl phosphatidylglycerol + H2O
-
 Naja melanoleuca ?
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O inflammatory diseases Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins Sus scrofa ?
-
 ?
3.1.1.4 rac-1,2-dihexanoyldithiopropyl-3-phosphocholine + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 monomer
-
 Homo sapiens
3.1.1.4 monomer
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 20
-
 after 7 days more than 95% of the activity remains, in presence or absence of Ca2+, pH ranging from 4.5 to 9.0 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 8
-
-
 Homo sapiens
3.1.1.4 8
-
-
 Sus scrofa