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Literature summary extracted from

  • Tojo, H.; Ying, Z.; Okamoto, M.
    Purification and characterization of guinea pig gastric phospholipase A2 of the pancreatic type (1993), Eur. J. Biochem., 215, 81-90.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 anti-peptide antibody
-
Cavia porcellus
3.1.1.4 EGTA
-
Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 secretory granule
-
Cavia porcellus 30141
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 13900
-
HPLC-electrospray mass spectrometry Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Cavia porcellus
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 reverse-phase HPLC, gel filtration, from gastric juice, 180fold Cavia porcellus
3.1.1.4 reverse-phase HPLC, gel filtration, from gastric mucosa, 51.5fold Cavia porcellus
3.1.1.4 reverse-phase HPLC, gel filtration, from pancreas, more than 34.7fold Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 pancreas
-
Cavia porcellus
-
3.1.1.4 stomach gastric mucosa and juices Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 0.12
-
pancreas, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate Cavia porcellus
3.1.1.4 0.211
-
pancreas, substrate: 1-O-hexadecyl-oleolyl-sn-glycero-3-phosphocholine Cavia porcellus
3.1.1.4 0.45
-
antrum, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate Cavia porcellus
3.1.1.4 8.4
-
gastric juice, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate Cavia porcellus
3.1.1.4 20
-
fundus, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate Cavia porcellus
3.1.1.4 1000
-
gastric juice, substrate: 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine Cavia porcellus
3.1.1.4 1030
-
gastric mucosa, substrate: 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-O-hexadecyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O
-
Cavia porcellus 1-O-hexadecyl-sn-glycero-3-phosphocholine + oleate
-
?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine + H2O
-
Cavia porcellus 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol + H2O
-
?
3.1.1.4 phospholipids + H2O
-
Cavia porcellus ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 7 9 gastric PLA2, no activity detected below pH 5.0 Cavia porcellus