BRENDA - Enzyme Database

Cytosolic phospholipase A2 from human monocytic cells: characterization of substrate specificity and Ca2+-dependent membrane association

Rehfeldt, W.; Resch, K.; Goppelt-Struebe, M.; Biochem. J. 293, 255-261 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
phosphate
phosphorylation needed for activity
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
2-mercaptoethanol
-
Homo sapiens
3.1.1.4
EDTA
-
Homo sapiens
3.1.1.4
EGTA
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
cytoplasm
-
Homo sapiens
5737
-
3.1.1.4
plasma membrane
-
Homo sapiens
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
Ca2+-dependent PLA2-association with cellular membranes at a concentration of 300-500 nM
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
78000
-
native PAGE
Homo sapiens
3.1.1.4
100000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Homo sapiens
eicosanoids
?
-
-
?
3.1.1.4
phospholipids + H2O
Homo sapiens
inflammatory diseases
?
-
-
?
3.1.1.4
phospholipids + H2O
Homo sapiens
release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
column chromatography, gel electrophoresis, 1206fold
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
leukocyte
peripheral blood
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
0.0609
-
substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.4
-70°C, with bovine serum albumin, loss of enzyme activity only partially avoided
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O
-
133888
Homo sapiens
1-acyl-sn-glycerophosphoethanolamine + arachidonic acid
-
133888
Homo sapiens
?
3.1.1.4
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
133888
Homo sapiens
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylcholine + arachidonic acid
-
133888
Homo sapiens
1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine + carboxylic acid
-
133888
Homo sapiens
r
3.1.1.4
phosphatidylcholine + H2O
-
133888
Homo sapiens
1-acylglycerophosphocholine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylcholine + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylcholine
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylethanolamine + arachidonic acid
-
133888
Homo sapiens
arachidonoyl phosphatidylethanolamine
-
133888
Homo sapiens
r
3.1.1.4
phosphatidylethanolamine + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylethanolamine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylinositol + arachidonic acid
-
133888
Homo sapiens
arachidonoyl phosphatidylinositol + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylinositol + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylinositol + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylserine + arachidonic acid
-
133888
Homo sapiens
arachidonoyl phosphatidylserine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylserine + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylserine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phospholipids + H2O
eicosanoids
133888
Homo sapiens
?
-
-
-
?
3.1.1.4
phospholipids + H2O
inflammatory diseases
133888
Homo sapiens
?
-
-
-
?
3.1.1.4
phospholipids + H2O
release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins
133888
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.4
monomer
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
phosphate
phosphorylation needed for activity
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
2-mercaptoethanol
-
Homo sapiens
3.1.1.4
EDTA
-
Homo sapiens
3.1.1.4
EGTA
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
cytoplasm
-
Homo sapiens
5737
-
3.1.1.4
plasma membrane
-
Homo sapiens
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
Ca2+-dependent PLA2-association with cellular membranes at a concentration of 300-500 nM
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
78000
-
native PAGE
Homo sapiens
3.1.1.4
100000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Homo sapiens
eicosanoids
?
-
-
?
3.1.1.4
phospholipids + H2O
Homo sapiens
inflammatory diseases
?
-
-
?
3.1.1.4
phospholipids + H2O
Homo sapiens
release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
column chromatography, gel electrophoresis, 1206fold
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
leukocyte
peripheral blood
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
0.0609
-
substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.4
-70°C, with bovine serum albumin, loss of enzyme activity only partially avoided
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O
-
133888
Homo sapiens
1-acyl-sn-glycerophosphoethanolamine + arachidonic acid
-
133888
Homo sapiens
?
3.1.1.4
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
133888
Homo sapiens
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylcholine + arachidonic acid
-
133888
Homo sapiens
1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine + carboxylic acid
-
133888
Homo sapiens
r
3.1.1.4
phosphatidylcholine + H2O
-
133888
Homo sapiens
1-acylglycerophosphocholine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylcholine + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylcholine
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylethanolamine + arachidonic acid
-
133888
Homo sapiens
arachidonoyl phosphatidylethanolamine
-
133888
Homo sapiens
r
3.1.1.4
phosphatidylethanolamine + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylethanolamine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylinositol + arachidonic acid
-
133888
Homo sapiens
arachidonoyl phosphatidylinositol + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylinositol + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylinositol + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylserine + arachidonic acid
-
133888
Homo sapiens
arachidonoyl phosphatidylserine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phosphatidylserine + linoleic acid
-
133888
Homo sapiens
lineoyl phosphatidylserine + fatty acid
-
133888
Homo sapiens
?
3.1.1.4
phospholipids + H2O
eicosanoids
133888
Homo sapiens
?
-
-
-
?
3.1.1.4
phospholipids + H2O
inflammatory diseases
133888
Homo sapiens
?
-
-
-
?
3.1.1.4
phospholipids + H2O
release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins
133888
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.4
monomer
-
Homo sapiens