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Literature summary extracted from

  • Nok, A.J.; Esievo, K.A.N.; Ibrahim, S.; Ukoha, A.I.; Ikediobi, C.O.
    Phospholipase A2 from Trypanosoma congolense: characterization and haematological properties (1993), Cell Biochem. Funct., 11, 125-130.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 EDTA
-
 Trypanosoma congolense
3.1.1.4 p-hydroxymercuribenzoate
-
 Trypanosoma congolense
3.1.1.4 tributyltin oxide
-
 Trypanosoma congolense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 1.85
-
 dimyristoyl-sn-phosphatidylcholine
-
 Trypanosoma congolense
3.1.1.4 2.12
-
 dioleolyl-sn-phosphatidylcholine
-
 Trypanosoma congolense
3.1.1.4 3.65
-
 dimyristoyl-sn-phosphatidylserine
-
 Trypanosoma congolense
3.1.1.4 4.6
-
 dioleolyl-sn-phosphatidylethanolamine
-
 Trypanosoma congolense
3.1.1.4 56
-
 dipalmitoyl-sn-phosphatidylserine
-
 Trypanosoma congolense
3.1.1.4 58
-
 dimyristoyl-sn-phosphatidylinositol
-
 Trypanosoma congolense
3.1.1.4 86
-
 dipalmitoyl-phosphatidylcholine
-
 Trypanosoma congolense
3.1.1.4 98
-
 dipalmitoyl-phosphatidylethanolamine
-
 Trypanosoma congolense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Trypanosoma congolense
3.1.1.4 Zn2+
-
 Trypanosoma congolense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 16500
-
 1 * 16500 + 1 * 18000, SDS-PAGE Trypanosoma congolense
3.1.1.4 18000
-
 1 * 16500 + 1 * 18000, SDS-PAGE Trypanosoma congolense
3.1.1.4 34500
-
 dimer with a 16500 and a 18000 subunit, SDS-PAGE Trypanosoma congolense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Trypanosoma congolense hemolytic activity of basic PLA2 ?
-
 ?
3.1.1.4 phospholipids + H2O Trypanosoma congolense influence on platelet aggregation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Trypanosoma congolense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 ion exchange chromatography Trypanosoma congolense

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 4°C, high stability even at low concentrations, i.e. 50 mg,in 20 mM phosphate buffer pH 6.8 containing 2 mM of 2-mercaptoethanol Trypanosoma congolense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
 Trypanosoma congolense 1-myristoyl-phosphorylcholine + myristic acid
-
 ?
3.1.1.4 1,2-dimyristoyl-sn-phosphatidylinositol + H2O
-
 Trypanosoma congolense 1-myristoyl-sn-phosphatidylinositol + myristic acid
-
 ?
3.1.1.4 1,2-dimyristoyl-sn-phosphatidylserine + H2O
-
 Trypanosoma congolense 1-myristoyl-sn-phosphatidylserine + myristic acid
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
 Trypanosoma congolense 1-oleoyl-sn-phosphatidylcholine + oleate
-
 ?
3.1.1.4 1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
 Trypanosoma congolense 1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
 ?
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Trypanosoma congolense 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 1,2-dipalmitoyl-phosphatidylethanolamine + H2O
-
 Trypanosoma congolense 1-palmitoyl-phosphatidylethanolamine + palmitic acid
-
 ?
3.1.1.4 1,2-dipalmitoyl-sn-phosphatidylserine + H2O
-
 Trypanosoma congolense 1-palmitoyl-sn-phosphatidylserine + palmitic acid
-
 ?
3.1.1.4 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine + H2O
-
 Trypanosoma congolense 1-palmitoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid
-
 ?
3.1.1.4 dimyristoyl phosphatidylcholine + H2O
-
 Trypanosoma congolense 1-myristoyl-sn-glycerophosphocholine + myristic acid
-
 ?
3.1.1.4 dimyristoyl phosphatidylinositol + H2O
-
 Trypanosoma congolense 1-myristoyl-sn-glycerophosphoinositol + myristic acid
-
 ?
3.1.1.4 dimyristoyl phosphatidylserinel + H2O
-
 Trypanosoma congolense 1-myristoyl-sn-glycerophosphoserine + myristic acid
-
 ?
3.1.1.4 dioleolyl-sn- phosphatidylcholine + H2O
-
 Trypanosoma congolense 1-oleolyl-sn-glycerophosphocholine + oleic acid
-
 ?
3.1.1.4 dioleolyl-sn- phosphatidylethanolamine + H2O
-
 Trypanosoma congolense 1-oleolyl-sn-glycerophosphoethanolamine + oleic acid
-
 ?
3.1.1.4 dipalmitoyl- phosphatidylcholine + H2O
-
 Trypanosoma congolense 1-palmitoyl-sn-glycerophosphocholine + palmitic acid
-
 ?
3.1.1.4 dipalmitoyl- phosphatidylethanolamine + H2O
-
 Trypanosoma congolense 1-palmitoyl-sn-glycerophosphoethanolamine + palmitic acid
-
 ?
3.1.1.4 phospholipids + H2O hemolytic activity of basic PLA2 Trypanosoma congolense ?
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Trypanosoma congolense ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 dimer 1 * 16500 + 1 * 18000, SDS-PAGE Trypanosoma congolense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 35
-
-
 Trypanosoma congolense

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 50
-
 enzyme retains 70% of activity after 10 min incubation at 50°C, inactivation above 50°C Trypanosoma congolense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 6.5 6.8
-
 Trypanosoma congolense