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Literature summary extracted from

  • Kawauchi, Y.; Takasaki, J.; Matsuura, Y.; Masuho, Y.
    Preparation and characterization of human rheumatoid arthritic synovial fluid phospholipase A2 produced by recombinant baculovirus-infected cells (1994), J. Biochem., 116, 82-87.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 into pAcYM1, transfer into baculovirus, transfection of Sf9 S.frugiperda cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 Ca2+
-
 Homo sapiens
3.1.1.4 sodium deoxycholate
-
 Homo sapiens
3.1.1.4 Triton X-100
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.02
-
 phosphatidylethanolamine recombinant PLA2 Homo sapiens
3.1.1.4 0.024
-
 phosphatidylethanolamine natural PLA2 Homo sapiens
3.1.1.4 0.05
-
 phosphatidylcholine recombinant PLA2 Homo sapiens
3.1.1.4 0.051
-
 phosphatidylcholine natural PLA2 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 13700
-
 natural PLA2, SDS-PAGE Homo sapiens
3.1.1.4 14100
-
 recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Homo sapiens inflammatory diseases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 cation exchange column, reversed phase HPLC Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 synovia
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 110
-
 recombinant enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine Homo sapiens
3.1.1.4 221
-
 natural enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
 Homo sapiens 1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
 ?
3.1.1.4 phosphatidylethanolamine + H2O
-
 Homo sapiens 1-acylglycerophosphorylethanolamine + fatty acid
-
 ?
3.1.1.4 phospholipids + H2O inflammatory diseases Homo sapiens ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 6 10 optimum at pH 9 Homo sapiens