BRENDA - Enzyme Database

Preparation and characterization of human rheumatoid arthritic synovial fluid phospholipase A2 produced by recombinant baculovirus-infected cells

Kawauchi, Y.; Takasaki, J.; Matsuura, Y.; Masuho, Y.; J. Biochem. 116, 82-87 (1994)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.4
into pAcYM1, transfer into baculovirus, transfection of Sf9 S.frugiperda cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Homo sapiens
3.1.1.4
sodium deoxycholate
-
Homo sapiens
3.1.1.4
Triton X-100
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.02
-
phosphatidylethanolamine
recombinant PLA2
Homo sapiens
3.1.1.4
0.024
-
phosphatidylethanolamine
natural PLA2
Homo sapiens
3.1.1.4
0.05
-
phosphatidylcholine
recombinant PLA2
Homo sapiens
3.1.1.4
0.051
-
phosphatidylcholine
natural PLA2
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
13700
-
natural PLA2, SDS-PAGE
Homo sapiens
3.1.1.4
14100
-
recombinant enzyme, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Homo sapiens
inflammatory diseases
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
cation exchange column, reversed phase HPLC
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
synovia
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
110
-
recombinant enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
Homo sapiens
3.1.1.4
221
-
natural enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
133881
Homo sapiens
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
133881
Homo sapiens
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133881
Homo sapiens
1-acylglycerophosphorylethanolamine + fatty acid
-
133881
Homo sapiens
?
3.1.1.4
phospholipids + H2O
inflammatory diseases
133881
Homo sapiens
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
6
10
optimum at pH 9
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
into pAcYM1, transfer into baculovirus, transfection of Sf9 S.frugiperda cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Homo sapiens
3.1.1.4
sodium deoxycholate
-
Homo sapiens
3.1.1.4
Triton X-100
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.02
-
phosphatidylethanolamine
recombinant PLA2
Homo sapiens
3.1.1.4
0.024
-
phosphatidylethanolamine
natural PLA2
Homo sapiens
3.1.1.4
0.05
-
phosphatidylcholine
recombinant PLA2
Homo sapiens
3.1.1.4
0.051
-
phosphatidylcholine
natural PLA2
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
13700
-
natural PLA2, SDS-PAGE
Homo sapiens
3.1.1.4
14100
-
recombinant enzyme, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Homo sapiens
inflammatory diseases
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
cation exchange column, reversed phase HPLC
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
synovia
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
110
-
recombinant enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
Homo sapiens
3.1.1.4
221
-
natural enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
133881
Homo sapiens
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
133881
Homo sapiens
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133881
Homo sapiens
1-acylglycerophosphorylethanolamine + fatty acid
-
133881
Homo sapiens
?
3.1.1.4
phospholipids + H2O
inflammatory diseases
133881
Homo sapiens
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
6
10
optimum at pH 9
Homo sapiens