BRENDA - Enzyme Database

Purification and characterization of nuclear alkaline phospholipase A2 in rat ascites hepatoma cells

Oishi, T.; Tamiya-Koizumi, K.; Kudo, I.; Iino, S.; Takagi, K.; Yoshida, Y.; FEBS Lett. 394, 55-60 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
anti-peptide antibody
group II PLA2-antibody
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.2
-
arachidonyl phosphatidylethanolamine
neutral nuclear PLA2
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
nucleus
in chromatin rich fraction
Rattus norvegicus
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
33000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Rattus norvegicus
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
column chromatography, immunoadsorbent column containing group II PLA2-antibodies, 735fold
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
hepatoma ascites cell line
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
133877
Rattus norvegicus
1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1,2-dipalmitoyl-sn-phosphatidylserine + H2O
-
133877
Rattus norvegicus
1-palmitoyl-sn-phosphatidylserine + palmitic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O
-
133877
Rattus norvegicus
1-acyl-sn-glycero-3-phosphoethanolamine + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O
-
133877
Rattus norvegicus
1-acyl-sn-glycerophosphoethanolamine + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
133877
Rattus norvegicus
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + H2O
-
133877
Rattus norvegicus
1-stearoyl-sn-glycero-3-phosphoinositol + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
-
133877
Rattus norvegicus
1-stearoyl-glycerophosphorylcholine + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133877
Rattus norvegicus
1-acylglycerophosphorylethanolamine + fatty acid
-
133877
Rattus norvegicus
?
3.1.1.4
phospholipids + H2O
-
133877
Rattus norvegicus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
9
10
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
anti-peptide antibody
group II PLA2-antibody
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
0.2
-
arachidonyl phosphatidylethanolamine
neutral nuclear PLA2
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.4
nucleus
in chromatin rich fraction
Rattus norvegicus
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
33000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Rattus norvegicus
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
column chromatography, immunoadsorbent column containing group II PLA2-antibodies, 735fold
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
hepatoma ascites cell line
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
133877
Rattus norvegicus
1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1,2-dipalmitoyl-sn-phosphatidylserine + H2O
-
133877
Rattus norvegicus
1-palmitoyl-sn-phosphatidylserine + palmitic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O
-
133877
Rattus norvegicus
1-acyl-sn-glycero-3-phosphoethanolamine + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O
-
133877
Rattus norvegicus
1-acyl-sn-glycerophosphoethanolamine + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
133877
Rattus norvegicus
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + H2O
-
133877
Rattus norvegicus
1-stearoyl-sn-glycero-3-phosphoinositol + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
-
133877
Rattus norvegicus
1-stearoyl-glycerophosphorylcholine + arachidonic acid
-
133877
Rattus norvegicus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133877
Rattus norvegicus
1-acylglycerophosphorylethanolamine + fatty acid
-
133877
Rattus norvegicus
?
3.1.1.4
phospholipids + H2O
-
133877
Rattus norvegicus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
9
10
-
Rattus norvegicus