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Literature summary extracted from

  • Brown, W.J.; Sgoutas, D.S.
    Purification of rat liver lysosomal cholesteryl ester hydrolase (1980), Biochim. Biophys. Acta, 617, 305-317.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.13 50% loss of activity after storage at 4°C, 2 weeks Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 5,5-dithiobis(2-nitrobenzoic acid) 80% inhibition at 10 mM Rattus sp.
3.1.1.13 Ca2+ complete inactivation at 10 mM Rattus sp.
3.1.1.13 Mg2+ 44% inhibition at 5 mM Rattus sp.
3.1.1.13 N-ethylmaleimide 72% inhibition at 3 mM Rattus sp.
3.1.1.13 NaF 65% inhibition at 5 mM Rattus sp.
3.1.1.13 p-chloromercuribenzoate 76% inhibition at 1 mM Rattus sp.
3.1.1.13 Protamine sulfate 18% inhibition at 500 mg/ml Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.13 lysosome
-
 Rattus sp. 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 60000
-
 gel filtration, SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Rattus sp. hydrolytic activity toward liposomal and microcrystallic substrates cholesterol + fatty acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13 90% purity by digitonin solubilization of lysosome-enriched pellets, chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 0.018
-
 for p-nitrophenylacetate Rattus sp.
3.1.1.13 0.31
-
 for p-nitrophenylmyristate Rattus sp.

Storage Stability

EC Number Storage Stability Organism
3.1.1.13 4°C, several days Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O hydrolytic activity toward liposomal and microcrystallic substrates Rattus sp. cholesterol + fatty acid
-
 ?
3.1.1.13 cholesteryl oleate + H2O
-
 Rattus sp. cholesterol + oleic acid
-
 ?
3.1.1.13 p-nitrophenyl acetate + H2O
-
 Rattus sp. p-nitrophenol + acetic acid
-
 ?
3.1.1.13 p-nitrophenyllactate + H2O
-
 Rattus sp. p-nitrophenol + lactic acid
-
 ?
3.1.1.13 p-nitrophenylmyristate + H2O
-
 Rattus sp. p-nitrophenol + myristic acid
-
 ?
3.1.1.13 triolein + H2O
-
 Rattus sp. dioleoylglycerol + oleic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 monomer 1 * 60000, SDS-PAGE, gel filtration, also active as aggregates Rattus sp.