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Literature summary extracted from

  • Tuhackovaá, Z.; Kriz, O.; Hradec, J.
    Purification and some properties of a cholesterol esterase from rat liver (1980), Biochim. Biophys. Acta, 617, 439-445.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 sodium taurocholic acid absolutely required, glycocholate can fully be substituted Rattus sp.
3.1.1.13 Trihydroxy bile salts lead to conversion of enzyme tetramers into monomers Rattus sp.

General Stability

EC Number General Stability Organism
3.1.1.13 freezing and storage at -80°C deactivate, addition of bovine serum albumin increases activity Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 deoxycholate 0.1% v/v, hydrolysis not detectable Rattus sp.
3.1.1.13 N-ethylmaleimide complete loss of activity Rattus sp.
3.1.1.13 Triton X-100 0.1% v/v, 12% substrate hydrolyzed in the presence of bile salts Rattus sp.
3.1.1.13 Tween 40 0.1% v/v, hydrolysis not detectable Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.13 0.31
-
 cholesteryl oleate
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.13 cytosol
-
 Rattus sp. 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.13 K+
-
 Rattus sp.
3.1.1.13 Mg2+
-
 Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 65000
-
 SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Rattus sp.
-
 cholesterol + fatty acid
-
 ir

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.13 Acetone reduces hydrolysis rate significantly Rattus sp.
3.1.1.13 Glycerol inactivates Rattus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13 3step chromatography techniques, preparative electrophoresis to homogeneity Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 0.34
-
-
 Rattus sp.

Storage Stability

EC Number Storage Stability Organism
3.1.1.13 0°C, 1 week Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O
-
 Rattus sp. cholesterol + fatty acid
-
 ir
3.1.1.13 cholesteryl oleate + H2O
-
 Rattus sp. cholesterol + oleic acid
-
 ir

Subunits

EC Number Subunits Comment Organism
3.1.1.13 tetramer 4 * 65000, native PAGE, SDS-PAGE, activity also in monomers Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 7 7.5
-
 Rattus sp.