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Literature summary extracted from

  • Negre, A.; Salvayre, R.; Rogalle, P.; Dang, Q.Q.; Douste-Blazy, L.
    Acyl-chain specificity and properties of cholesterol esterases from normal and Wolman lymphoid cell lines (1987), Biochim. Biophys. Acta, 918, 76-82.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 sodium taurocholic acid optimal concentration for acid cholesteryl esterase at 2.5-5.0 mM/l, for neutral cholesteryl esterase at 0.1-1.0 mM/l Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 dithiothreitol for neutral cholesterol esterase with pH optimum at 6.0 and for acid cholesterol esterase Homo sapiens
3.1.1.13 E600 strong for neutral cholesterol esterases Homo sapiens
3.1.1.13 EDTA inhibition only for neutral cholesterol esterases Homo sapiens
3.1.1.13 Hg2+ strong for acid and less for neutral cholesterol esterases Homo sapiens
3.1.1.13 iodoacetate acid and neutral cholesterol esterases Homo sapiens
3.1.1.13 N-ethylmaleimide for acid and neutral cholesterol esterases Homo sapiens
3.1.1.13 NaCl acid and neutral cholesterol esterases Homo sapiens
3.1.1.13 p-hydroxymercuribenzoate strongest inactivation for acid cholesterol esterase, modest for neutral cholesterol esterase with pH optimum 6.0 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.13 Ca2+ activation for neutral cholesterol esterases Homo sapiens
3.1.1.13 Mg2+ activation for neutral cholesterol esterases Homo sapiens
3.1.1.13 Mn2+ activation for neutral cholesterol esterases Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Homo sapiens acid cholesterol esterase hydrolized only long-chain cholesteryl esters, activity is low for substrate concentration below the critical micelle concentration cholesterol + fatty acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 lymphoid tissue
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl acetate + H2O very slowly hydrolyzed by acid cholesterol esterase, only hydrolyzed by neutral cholestrol esterase at pH 8.0 Homo sapiens cholesterol + acetic acid
-
 ?
3.1.1.13 cholesteryl butyrate + H2O very slowly hydrolyzed by acid cholesterol esterase, only hydrolyzed by neutral cholesterol esterase at pH 8.0 Homo sapiens cholesterol + butanoic acid
-
 ?
3.1.1.13 cholesteryl caprylate + H2O
-
 Homo sapiens cholesterol + caprylic acid
-
 ?
3.1.1.13 cholesteryl esters + H2O acid cholesterol esterase hydrolized only long-chain cholesteryl esters, activity is low for substrate concentration below the critical micelle concentration Homo sapiens cholesterol + fatty acid
-
 ?
3.1.1.13 cholesteryl laurate + H2O
-
 Homo sapiens cholesterol + lauric acid
-
 ?
3.1.1.13 cholesteryl oleate + H2O
-
 Homo sapiens cholesterol + oleic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.13 acid cholesterol esterase
-
 Homo sapiens
3.1.1.13 neutral cholesterol esterase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.13 55
-
 discriminated at acid pH, neutral cholesterol esterases more heat-labile than acid cholesterol esterase Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 4
-
 for acid cholesterol esterase Homo sapiens
3.1.1.13 6
-
 for neutral cholesterol esterase I Homo sapiens
3.1.1.13 8
-
 for neutral cholesterol esterase II Homo sapiens