Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • DiPersio, L.P.; Kissel, J.A.; Hui, D.Y.
    Purification of pancreatic cholesterol esterase expressed in recombinant baculovirus-infected Sf9 cells (1992), Protein Expr. Purif., 3, 114-120.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 sodium taurocholic acid
-
Rattus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.13 overexpression in baculovirus-infected Sf9 cells Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 64000
-
gel filtration, SDS-PAGE, recombinant protein after 5-day incubation period Rattus sp.
3.1.1.13 74000
-
SDS-PAGE, recombinant protein Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Rattus sp. mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats cholesterol + fatty acid
-
?
3.1.1.13 cholesteryl esters + H2O Rattus sp. important role in fat digestion in infants cholesterol + fatty acid
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13 overexpression in Sf9 cells, heparin-Sepharose chromatography, gel filtration Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 pancreas
-
Rattus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats Rattus sp. cholesterol + fatty acid
-
?
3.1.1.13 cholesteryl esters + H2O important role in fat digestion in infants Rattus sp. cholesterol + fatty acid
-
?
3.1.1.13 p-nitrophenyl butyrate + H2O
-
Rattus sp. p-nitrophenol + butanoic acid
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 monomer 1 * 74000, SDS-PAGE Rattus sp.
3.1.1.13 monomer 1 * 64000, gel filtration, SDS-PAGE Rattus sp.