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Literature summary extracted from

  • Batie, C.J.; LaHaie, E.; Ballou, D.P.
    Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia (1987), J. Biol. Chem., 262, 1510-1518.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.12.7 1,10-phenanthroline
-
Burkholderia cepacia
1.14.12.7 Cu2+
-
Burkholderia cepacia
1.14.12.7 EDTA
-
Burkholderia cepacia
1.14.12.7 Zn2+
-
Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.7 Fe3+ stimulates Burkholderia cepacia
1.14.12.7 Iron active form of enzyme contains one mononuclear iron and one [2Fe-2S] center per monomer Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.7 48000
-
4 * 48000, SDS-PAGE Burkholderia cepacia
1.14.12.7 217000
-
gel filtration Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.64 cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ Burkholderia cepacia involved in the phthalate degradation pathway in bacteria 4,5-dihydroxyphthalate + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.64 Burkholderia cepacia
-
-
-
1.14.12.7 Burkholderia cepacia
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.64 cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+
-
Burkholderia cepacia 4,5-dihydroxyphthalate + NADH + H+
-
?
1.3.1.64 cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ involved in the phthalate degradation pathway in bacteria Burkholderia cepacia 4,5-dihydroxyphthalate + NADH + H+
-
?
1.14.12.7 2,3-dicarboxypyridine + NADH + O2 69% of the activity with o-phthalate Burkholderia cepacia 5,6-dihydroxy-5,6-dihydropyridine-2,3-dicarboxylate + NAD+ + H2O
-
?
1.14.12.7 4-chloro-o-phthalate + NADH + O2 79% of the activity with o-phthalate Burkholderia cepacia 4-chloro-cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
?
1.14.12.7 phthalate + NADH + O2 o-phthalate Burkholderia cepacia cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.12.7 tetramer 4 * 48000, SDS-PAGE Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.64 NAD+
-
Burkholderia cepacia
1.3.1.64 NADH
-
Burkholderia cepacia
1.14.12.7 FMN enzyme contains FMN Burkholderia cepacia
1.14.12.7 NADH
-
Burkholderia cepacia