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Literature summary extracted from

  • Eisenberg, M.A.; Krell, K.
    Dethiobiotin synthetase (1979), Methods Enzymol., 62, 348-352.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.3 ADP competitive to ATP Escherichia coli
6.3.3.3 dethiobiotin 10 mM, 30% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.3 0.0013
-
 7,8-diaminononanoate
-
 Escherichia coli
6.3.3.3 0.005
-
 ATP
-
 Escherichia coli
6.3.3.3 0.6
-
 Mg2+
-
 Escherichia coli
6.3.3.3 3.4
-
 CO2
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.3.3 Mg2+ required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.3 24500
-
 2 * 24500, SDS-PAGE Escherichia coli
6.3.3.3 42000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.3
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.3 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.3.3.3 -20°C, 90% loss of activity after 24 h Escherichia coli
6.3.3.3 4°C, stable for about 2 weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 CO2 in form of HCO3- Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + diaminobiotin + CO2
-
 Escherichia coli ADP + phosphate + biotin
-
 ?
6.3.3.3 CTP + 7,8-diaminononanoate + CO2 50% of the activity relative to ATP Escherichia coli CDP + phosphate + dethiobiotin
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.3.3 dimer 2 * 24500, SDS-PAGE Escherichia coli