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Literature summary extracted from

  • Hack, E.; Kemp, J.D.
    Purification and characterization of the crown gall-specific enzyme, octopine synthase (1980), Plant Physiol., 65, 949-955.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.11 epsilon-aminohexanoate
-
 Helianthus annuus
1.5.1.11 NADP+ uncompetitive with respect to pyruvate Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.11 1.85
-
 L-Orn
-
 Helianthus annuus
1.5.1.11 8.8
-
 L-Arg
-
 Helianthus annuus
1.5.1.11 11.2
-
 L-Met
-
 Helianthus annuus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.11 38000
-
 1 * 38000, SDS-PAGE Helianthus annuus
1.5.1.11 39000
-
 gel filtration Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.11 Helianthus annuus
-
 crown-gall tumor-tissue induced by Agrobacterium tumefaciens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.11 crown-gall tumor-tissue induced by Agrobacterium tumefaciens Helianthus annuus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.11 N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+ partially ordered mechanism. NADPH binds first followed by the random order, rapid-equilibrium-binding of Arg and pyruvate. Orn probably binds before pyruvate Helianthus annuus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.11 crown gall induced by Agrobacterium tumefaciens Helianthus annuus
-
1.5.1.11 crown gall the structural gene for octopine synthase is located in a central portion of the T-DNA of Agrobacterium tumefaciens Ti plasmid Helianthus annuus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.11 additional information
-
-
 Helianthus annuus

Storage Stability

EC Number Storage Stability Organism
1.5.1.11 -30°C, buffer containing glycerol, NADPH and 2-mercaptoethanol, stable for many weeks Helianthus annuus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.11 L-Arg + pyruvate + NADH
-
 Helianthus annuus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
 ?
1.5.1.11 L-Arg + pyruvate + NADPH
-
 Helianthus annuus N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O
-
 ?
1.5.1.11 L-Gln + pyruvate + NADPH
-
 Helianthus annuus N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O
-
 ?
1.5.1.11 L-Lys + pyruvate + NADPH
-
 Helianthus annuus Lysopine + NADP+ + H2O
-
 ?
1.5.1.11 L-Met + pyruvate + NADPH
-
 Helianthus annuus N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O
-
 ?
1.5.1.11 Orn + pyruvate + NADH
-
 Helianthus annuus N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.11 monomer 1 * 38000, SDS-PAGE Helianthus annuus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.11 NADH
-
 Helianthus annuus
1.5.1.11 NADPH
-
 Helianthus annuus