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Literature summary extracted from

  • Gäde, G.; Carlsson, K.H.
    Purification and characterisation of octopine dehydrogenase from marine nemertean Cerebratulus lactus (anopla: heteronemerta): comparison with scallop octopine dehydrogenase (1984), Mar. Biol., 79, 39-45.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.11 octopine substrate inhibition above 2 mM, 50% product inhibition at 1.2 mM Cerebratulus lacteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.11 0.014
-
NAD+
-
Cerebratulus lacteus
1.5.1.11 0.028
-
NADH
-
Cerebratulus lacteus
1.5.1.11 0.22
-
octopine
-
Cerebratulus lacteus
1.5.1.11 0.5 1.5 pyruvate depending on concentration of the cosubstrates NADH and Arg Cerebratulus lacteus
1.5.1.11 1.4 2.8 Arg dependent on concentration of the cosubstrates NADH and pyruvate Cerebratulus lacteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.11 39000
-
ultracentrifugal studies Cerebratulus lacteus
1.5.1.11 41500
-
1 * 41500, SDS-PAGE Cerebratulus lacteus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.11 Cerebratulus lacteus
-
-
-
1.5.1.11 Pecten jacobaeus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.11
-
Cerebratulus lacteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.11 adductor
-
Pecten jacobaeus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.11 additional information
-
-
Cerebratulus lacteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.11 Canavanine + pyruvate + NADH L-canavanine, at 56% of the activity with L-Arg Cerebratulus lacteus N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
?
1.5.1.11 Canavanine + pyruvate + NADH L-canavanine, at 48% of the activity with L-Arg Pecten jacobaeus N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
?
1.5.1.11 Homoarginine + pyruvate + NADH at 22% of the activity with L-Arg Pecten jacobaeus N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
?
1.5.1.11 Homoarginine + pyruvate + NADH at 13% of the activity with L-Arg Cerebratulus lacteus N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
?
1.5.1.11 L-Arg + 2-oxobutanoate + NADH at 41% of the activity with L-Arg Cerebratulus lacteus N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
?
1.5.1.11 L-Arg + 2-oxobutanoate + NADH at 28% of the activity with pyruvate Pecten jacobaeus N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
?
1.5.1.11 L-Arg + pyruvate + NADH
-
Pecten jacobaeus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
?
1.5.1.11 L-Arg + pyruvate + NADH r Cerebratulus lacteus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
?
1.5.1.11 L-Lys + pyruvate + NADH at 2% of the activity with L-Arg Pecten jacobaeus Lysopine + NAD+ + H2O
-
?
1.5.1.11 L-Lys + pyruvate + NADH at 1% of the activity with L-Arg Cerebratulus lacteus Lysopine + NAD+ + H2O
-
?
1.5.1.11 Orn + pyruvate + NADH at 2% of the activity with L-Arg Cerebratulus lacteus N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
?
1.5.1.11 Orn + pyruvate + NADH at 4% of the activity with L-Arg Pecten jacobaeus N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.11 monomer 1 * 41500, SDS-PAGE Cerebratulus lacteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.11 7
-
-
Cerebratulus lacteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.11 6 8 about 70% of maximal activity at pH 6 and pH 8 Cerebratulus lacteus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.11 NADH
-
Pecten jacobaeus
1.5.1.11 NADH
-
Cerebratulus lacteus