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Literature summary extracted from

  • Grimshaw, C.E.; Henderson, G.B.; Soppe, G.G.; Hansen, G.; Mathur, E.J.; Huennekens, F.M.
    Purification and properties of 5,10-methenyltetrahydrofolate synthetase from Lactobacillus casei (1984), J. Biol. Chem., 259, 2728-2733.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.2 Cl-
-
 Lacticaseibacillus casei
6.3.3.2 Maleate
-
 Lacticaseibacillus casei
6.3.3.2 oxalate
-
 Lacticaseibacillus casei
6.3.3.2 phosphate
-
 Lacticaseibacillus casei
6.3.3.2 SO42-
-
 Lacticaseibacillus casei
6.3.3.2 succinate
-
 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.2 0.00021
-
 ATPgammaS
-
 Lacticaseibacillus casei
6.3.3.2 0.0006
-
 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei
6.3.3.2 0.00096
-
 ATP
-
 Lacticaseibacillus casei
6.3.3.2 0.001
-
 ATP
-
 Lacticaseibacillus casei
6.3.3.2 0.0013
-
 dATP
-
 Lacticaseibacillus casei
6.3.3.2 0.0016
-
 CTP
-
 Lacticaseibacillus casei
6.3.3.2 0.0056
-
 araATP
-
 Lacticaseibacillus casei
6.3.3.2 0.0064
-
 UTP
-
 Lacticaseibacillus casei
6.3.3.2 0.0066
-
 dTTP
-
 Lacticaseibacillus casei
6.3.3.2 0.021
-
 GTP
-
 Lacticaseibacillus casei
6.3.3.2 0.024
-
 ITP
-
 Lacticaseibacillus casei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.3.2 Mg2+ no divalent metal ion dependence beyond that required in the MgATP2- substrate complex Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.2 23000
-
 gel filtration Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.2 3.8
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 araATP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei araADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Lacticaseibacillus casei ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
6.3.3.2 ATPgammaS + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei ADPgammaS + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 CTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei CDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 dATP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei dADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 dTTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei dTDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 GTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei GDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 ITP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei IDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 UTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei UDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.3.2 monomer 1 * 23000, SDS-PAGE Lacticaseibacillus casei