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Literature summary extracted from

  • Hopkins, S.; Schirch, V.
    5,10-Methenyltetrahydrofolate synthetase. Purification and properties of the enzyme from rabbit liver (1984), J. Biol. Chem., 259, 5618-5622.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.2 (NH4)2SO4
-
 Oryctolagus cuniculus
6.3.3.2 5-methyltetrahydrofolate
-
 Oryctolagus cuniculus
6.3.3.2 AMP
-
 Oryctolagus cuniculus
6.3.3.2 MgADP-
-
 Oryctolagus cuniculus
6.3.3.2 NaCl
-
 Oryctolagus cuniculus
6.3.3.2 phosphate
-
 Oryctolagus cuniculus
6.3.3.2 SO42- Na2SO4 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.2 0.0005
-
 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus
6.3.3.2 0.3
-
 ATP Km-value is constant over the pH-range 5.0-7.5 Oryctolagus cuniculus
6.3.3.2 0.4
-
 CTP
-
 Oryctolagus cuniculus
6.3.3.2 0.5
-
 UTP
-
 Oryctolagus cuniculus
6.3.3.2 0.6
-
 CTP
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.3.2 Ca2+ as effective as Mg2+ Oryctolagus cuniculus
6.3.3.2 Co2+ slightly more effective than Mg2+ Oryctolagus cuniculus
6.3.3.2 Mg2+ required Oryctolagus cuniculus
6.3.3.2 Mn2+ as effective as Mg2+ Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.2 25000
-
 1 * 25000, SDS-PAGE Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2
-
 Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.3.2 ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate substrate binds to the enzyme by a random mechanism, Cleland-type mechanism Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.3.2 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.2 additional information
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Oryctolagus cuniculus ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
6.3.3.2 CTP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus CDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 CTP + 5-formyltetrahydrofolate CTP in form of MgCTP2- Oryctolagus cuniculus CDP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
6.3.3.2 GTP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus GDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 UTP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus UDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 UTP + 5-formyltetrahydrofolate UTP in form of MgUTP2- Oryctolagus cuniculus UDP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.3.2 monomer 1 * 25000, SDS-PAGE Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.2 additional information
-
 additional information
-
 Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.3.2 6.5 7.5 below pH 6.5 and above pH 7.5, 4°C, in presence of 2-mercaptoethanol, 24 h, 90% loss of activity Oryctolagus cuniculus
6.3.3.2 7
-
 4°C, 2-mercaptoethanol, 24 h, 10% loss of activity Oryctolagus cuniculus