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Literature summary extracted from

  • Schrimsher, J.L.; Schendel, F.J.; Stubbe, J.
    Isolation of a multifunctional protein with aminoimidazole ribonucleotide synthetase, glycinamide ribonucleotide synthetase, and glycinamide ribonucleotide transformylase activities: characterization of aminoimidazole ribonucleotide synthetase (1986), Biochemistry, 25, 4356-4365.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.13 on repeated freeze-thawing activity is lost due to the precipitation of protein Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.1 0.009
-
 2-(formamido)-N1-(5-phosphoribosyl)acetamidine
-
 Gallus gallus
6.3.3.1 0.078
-
 ATP
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.3.1 K+ absolutely required Gallus gallus
6.3.3.1 K+ optimal concentration: 150 mM Gallus gallus
6.3.3.1 Mg2+ optimal concentration: 20 mM Gallus gallus
6.3.3.1 Mg2+ absolutely required Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.1 133000
-
 sucrose density gradient ultracentrifugation, trifunctional enzyme: AIR synthase, glycinamide ribonucleotide synthetase, and glycinamide ribonucleotide transformylase Gallus gallus
6.3.3.1 330000
-
 gel filtration, trifunctional enzyme: aminoimidazole ribonucleotide synthase, glycinamide ribonucleotide synthetase, and glycineamide ribonucleotide transformylase Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.1 Gallus gallus
-
 trifunctional enzyme with AIR synthetase activity, phosphoribosylglycinamide synthetase activity, and phosphoribosylglycinamide transformylase activity
-
6.3.4.13 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.1 trifunctional enzyme with AIR synthetase activity, phosphoribosylglycinamide synthetase activity, and phosphoribosylglycinamide transformylase activity Gallus gallus
6.3.4.13 copurification of phosphoribosylamine-glycine ligase and glycinamide ribonucleotide transformylase Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.3.1 liver
-
 Gallus gallus
-
6.3.4.13 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.1 0.43
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
6.3.3.1 -20°C, stable for many weeks Gallus gallus
6.3.4.13 -20°C, stable for many weeks Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.1 ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine ATP in form of MgATP2- Gallus gallus ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
-
 ?
6.3.3.1 ATP + 2-(formamido)-N1-(5-phosphoribosyl)acetamidine ir Gallus gallus ADP + phosphate + 1-(5-phosphoribosyl)-5-aminoimidazole
-
 ?
6.3.4.13 ATP + 5-phosphoribosylamine + Gly
-
 Gallus gallus ADP + phosphate + 5-phosphoribosylglycinamide
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.13 additional information
-
 additional information
-
 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.3.1 4.7
-
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.3.1 7.4 8.1 about 90% of maximal activity at pH 7.4 and 8.1 Gallus gallus