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Literature summary extracted from

  • Gubler, M.; Appold, Y.; Keck, W.
    Overexpression, purification and characterization of UDP-N-acetylmuramyl:L-alanine ligase from Escherichia coli (1996), J. Bacteriol., 178, 906-910.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.8 murC gene is amplified by PCR and cloned with the expression vector pTrc99A Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.8 3-chloro-L-Ala
-
 Escherichia coli
6.3.2.8 3-Cyano-L-Ala
-
 Escherichia coli
6.3.2.8 Gly
-
 Escherichia coli
6.3.2.8 KCl inhibition above 100 mM Escherichia coli
6.3.2.8 L-Ser
-
 Escherichia coli
6.3.2.8 NaCl inhibition above 100 mM Escherichia coli
6.3.2.8 UDP-N-acetylmuramoyl-L-Ala
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.8 0.058
-
 UDP-N-acetylmuramate
-
 Escherichia coli
6.3.2.8 0.087
-
 L-Ala
-
 Escherichia coli
6.3.2.8 0.092
-
 ATP
-
 Escherichia coli
6.3.2.8 0.173
-
 beta-chloro-L-Ala
-
 Escherichia coli
6.3.2.8 1.99
-
 L-Ser
-
 Escherichia coli
6.3.2.8 7.89
-
 Gly
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.8 (NH4)2SO4 stimulation by 5-25 mM Escherichia coli
6.3.2.8 Mg2+ divalent cation required, Mg2+ or Mn2+ Escherichia coli
6.3.2.8 Mg2+ optimal concentration: 20 mM Escherichia coli
6.3.2.8 Mn2+ divalent cation required Escherichia coli
6.3.2.8 Mn2+ optimal concentration: 5 mM, reaching 50% of the activity measured in the presence of 20 mM MgCl2 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.8 Escherichia coli
-
 murC gene is amplified by PCR and cloned in the expression vector pTrc99A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.8
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.8 ATP + UDP-N-acetylmuramate + beta-chloro-L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-beta-chloro-L-Ala
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + DL-Ser
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + Gly
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-serine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.8 6.85
-
 L-Ala
-
 Escherichia coli
6.3.2.8 11.1
-
 beta-chloro-L-Ala
-
 Escherichia coli
6.3.2.8 11.4
-
 L-Ser
-
 Escherichia coli
6.3.2.8 11.7
-
 Gly
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.8 9
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.8 7.5 10 7.5: about 70% of maximal activity, 10.0: about 95% of maximal activity Escherichia coli