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Literature summary extracted from

  • Liger, D.; Masson, A.; Blanot, D.; van Heijenoort, J.; Parquet, C.
    Study of the overproduced uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli (1996), Microb. Drug Resist., 2, 25-27.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.8 stability is strictly dependent on the presence of 2-mercaptoethanol Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.8 3-chloro-L-Ala
-
 Escherichia coli
6.3.2.8 3-Cyano-L-Ala
-
 Escherichia coli
6.3.2.8 3-Fluoro-L-Ala
-
 Escherichia coli
6.3.2.8 NEM partial protection by ATP Escherichia coli
6.3.2.8 p-hydroxymercuribenzoate partial protection by ATP Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.8 0.02
-
 L-Ala
-
 Escherichia coli
6.3.2.8 0.1
-
 UDP-N-acetylmuramate
-
 Escherichia coli
6.3.2.8 0.45
-
 ATP
-
 Escherichia coli
6.3.2.8 0.85
-
 Ser
-
 Escherichia coli
6.3.2.8 2.5
-
 Gly
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.8 53630
-
 mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala Escherichia coli adds the first amino acid to the sugar moiety of the peptidoglycan precursor, catalyzing one essential step in cell wall biosynthesis ?
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala Escherichia coli formation of a acylphosphate intermediate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.8 Escherichia coli
-
 JM83(pAM1005), overproduced in a strain harboring a recombinant plasmid pAM1005 with murC gen under the control of the inducible promoter trc
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.8 ATP + UDP-N-acetylmuramate + DL-Ser
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + Gly
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala r Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala adds the first amino acid to the sugar moiety of the peptidoglycan precursor, catalyzing one essential step in cell wall biosynthesis Escherichia coli ?
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala formation of a acylphosphate intermediate Escherichia coli ?
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-serine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.8 additional information
-
 additional information
-
 Escherichia coli