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Literature summary extracted from

  • Bauer, G.; Lingens, F.
    Microbial metabolism of quinoline and related compounds. XV. Quinoline-4-carboxylic acid oxidoreductase from Agrobacterium spec. 1B: a molybdenum-containing enzyme (1992), Biol. Chem. Hoppe-Seyler, 373, 699-705.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.99.19 molybdopterin cytosine dinucleotide cofactor Agrobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.19 4-chloromercuribenzoate
-
 Agrobacterium sp.
1.3.99.19 4-Chloroquinoline
-
 Agrobacterium sp.
1.3.99.19 4-Methylquinoline
-
 Agrobacterium sp.
1.3.99.19 Acriflavine
-
 Agrobacterium sp.
1.3.99.19 CN-
-
 Agrobacterium sp.
1.3.99.19 methanol
-
 Agrobacterium sp.
1.3.99.19 Quinoline
-
 Agrobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.19 0.00008
-
 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride with quinoline-4-carboxylate as cosubstrate Agrobacterium sp.
1.3.99.19 0.0093
-
 Quinoline-4-carboxylate with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate Agrobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.99.19 Iron enzyme contains 8.3 g-atoms of iron and 7.2 mol of acid-labile sulfur per mol of enzyme Agrobacterium sp.
1.3.99.19 Molybdenum enzyme contains 2.1 mol of molybdenum per mol of enzyme Agrobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.19 21000
-
 or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.
1.3.99.19 35000
-
 or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.
1.3.99.19 85000
-
 or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.
1.3.99.19 320000
-
 gel filtration Agrobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.19 Agrobacterium sp.
-
 1B
-
1.3.99.19 Agrobacterium sp. 1B
-
 1B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.19
-
 Agrobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.19 3.3
-
-
 Agrobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.19 4-chloroquinoline + acceptor with 4% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. ?
-
 ?
1.3.99.19 4-chloroquinoline + acceptor with 4% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. 1B ?
-
 ?
1.3.99.19 4-methylquinoline + acceptor with 24% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. ?
-
 ?
1.3.99.19 4-methylquinoline + acceptor with 24% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. 1B ?
-
 ?
1.3.99.19 quinoline + acceptor with 34% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. ?
-
 ?
1.3.99.19 quinoline + acceptor with 34% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. 1B ?
-
 ?
1.3.99.19 quinoline-4-carboxylate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
-
 Agrobacterium sp. 2-oxo-1,2-dihydroquinoline-4-carboxylate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
 r
1.3.99.19 quinoline-4-carboxylate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
-
 Agrobacterium sp. 1B 2-oxo-1,2-dihydroquinoline-4-carboxylate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
 r
1.3.99.19 quinoline-4-carboxylate + acceptor acceptors: 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride, thionin, menadione, benzoquinone, 2,6-dichlorophenol-indophenol Agrobacterium sp. 2-oxo-1,2-dihydroquinoline-4-carboxylic acid + reduced acceptor
-
 ?
1.3.99.19 quinoline-4-carboxylate + acceptor acceptors: 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride, thionin, menadione, benzoquinone, 2,6-dichlorophenol-indophenol Agrobacterium sp. 1B 2-oxo-1,2-dihydroquinoline-4-carboxylic acid + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.19 trimer or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.19 6
-
-
 Agrobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.19 FAD enzyme contains 1.7 mol of FAD per mol of enzyme Agrobacterium sp.