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Literature summary extracted from
- XVIII. Purification and some properties of the molybdenum- and iron-containing quinaldic acid 4-oxidoreductase from Serratia marcescens 2CC-1 (1993), Biol. Chem. Hoppe-Seyler, 374, 363-376.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.3.99.18 | ethylene glycol, 0.1%, Triton X-10, 0.1%, and Tween, 0.01%, inactivate the enzyme within 1-2 days at 4 C | Serratia marcescens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.99.18 | Cu2+ | - |
Serratia marcescens |  |
| 1.3.99.18 | Hg2+ | - |
Serratia marcescens | |
| 1.3.99.18 | KCN | - |
Serratia marcescens | |
| 1.3.99.18 | methanol | - |
Serratia marcescens | |
| 1.3.99.18 | Mn2+ | - |
Serratia marcescens | |
| 1.3.99.18 | NaAsO2 | - |
Serratia marcescens | |
| 1.3.99.18 | p-hydroxymercuribenzoate | - |
Serratia marcescens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.99.18 | 0.085 | - |
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | with quinaldate as cosubstrate | Serratia marcescens | |
| 1.3.99.18 | 1.45 | - |
Quinaldate | with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate | Serratia marcescens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.99.18 | Iron | enzyme contains 2.3-3.7 gatom of iron per mol of enzyme | Serratia marcescens | |
| 1.3.99.18 | Molybdenum | enzyme contains 0.5-0.6 gatom of molybdenum per mol of enzyme | Serratia marcescens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.18 | 90000 | 100000 | gel filtration | Serratia marcescens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.18 | Quinaldate + acceptor | Serratia marcescens | inducible enzyme | ? | - |
? | |
| 1.3.99.18 | Quinaldate + acceptor | Serratia marcescens 2CC-1 | inducible enzyme | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.99.18 | Serratia marcescens | - |
2CC-1 | - |
| 1.3.99.18 | Serratia marcescens 2CC-1 | - |
2CC-1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.99.18 | - |
Serratia marcescens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.18 | 64.5 | - |
- |
Serratia marcescens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.99.18 | -80°C, 8 days, 25% loss of activity | Serratia marcescens |
| 1.3.99.18 | 4°C, 8 days, 30% loss of activity | Serratia marcescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.18 | nicotinic acid + acceptor | - |
Serratia marcescens | 6-hydroxynicotinic acid + reduced acceptor | - |
? | |
| 1.3.99.18 | nicotinic acid + acceptor | - |
Serratia marcescens 2CC-1 | 6-hydroxynicotinic acid + reduced acceptor | - |
? | |
| 1.3.99.18 | quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O | - |
Serratia marcescens | kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | - |
r | |
| 1.3.99.18 | quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O | - |
Serratia marcescens 2CC-1 | kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | - |
r | |
| 1.3.99.18 | quinaldate + acceptor | acceptor: K3[Fe(CN)6] | Serratia marcescens | kynurenate + reduced acceptor | - |
? | |
| 1.3.99.18 | quinaldate + acceptor | acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | Serratia marcescens | kynurenate + reduced acceptor | - |
? | |
| 1.3.99.18 | quinaldate + acceptor | acceptor: K3[Fe(CN)6] | Serratia marcescens 2CC-1 | kynurenate + reduced acceptor | - |
? | |
| 1.3.99.18 | quinaldate + acceptor | acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | Serratia marcescens 2CC-1 | kynurenate + reduced acceptor | - |
? | |
| 1.3.99.18 | Quinaldate + acceptor | inducible enzyme | Serratia marcescens | ? | - |
? | |
| 1.3.99.18 | Quinaldate + acceptor | inducible enzyme | Serratia marcescens 2CC-1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.99.18 | dimer | 1 * 18000-19000, beta, + 1 * 75000-80000, alpha, SDS-PAGE | Serratia marcescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.18 | 70 | - |
- |
Serratia marcescens |
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