BRENDA - Enzyme Database

XX. Quinaldic acid 4-oxidoreductase from Pseudomonas sp. AK-2 compared to other procaryotic molybdenum-containing hydroxylases

Sauter, M.; Tshisuaka, B.; Fetzner, S.; Lingens, F.; Biol. Chem. Hoppe-Seyler 374, 1037-1046 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.99.18
Acriflavine
-
Pseudomonas sp.
1.3.99.18
CoCl2
-
Pseudomonas sp.
1.3.99.18
Cu2+
-
Pseudomonas sp.
1.3.99.18
KCN
74% reactivation with S2-
Pseudomonas sp.
1.3.99.18
methanol
-
Pseudomonas sp.
1.3.99.18
Mn2+
-
Pseudomonas sp.
1.3.99.18
NaAsO2
-
Pseudomonas sp.
1.3.99.18
NiCl2
-
Pseudomonas sp.
1.3.99.18
p-hydroxymercuribenzoate
-
Pseudomonas sp.
1.3.99.18
Quinacrine
-
Pseudomonas sp.
1.3.99.18
ZnSO4
-
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.99.18
0.023
-
Quinaldate
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
Pseudomonas sp.
1.3.99.18
1.6
-
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
with quinaldate as cosubstrate
Pseudomonas sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.99.18
Iron
contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme
Pseudomonas sp.
1.3.99.18
Molybdenum
enzyme contains 1.6 g-atom per mol of enzyme
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.99.18
20000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
1.3.99.18
32000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
1.3.99.18
90000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
1.3.99.18
300000
-
gel filtration
Pseudomonas sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.99.18
Pseudomonas sp.
-
AK-2
-
1.3.99.18
Pseudomonas sp. AK-2
-
AK-2
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.99.18
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.18
7.82
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.99.18
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
1.3.99.18
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
1.3.99.18
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
1.3.99.18
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
1.3.99.18
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
1.3.99.18
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
1.3.99.18
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp.
?
-
-
-
-
1.3.99.18
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp. AK-2
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.3.99.18
hexamer
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.99.18
60
-
above 60°C
Pseudomonas sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.99.18
75
-
5 min, 32% loss of activity
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.99.18
8
-
-
Pseudomonas sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.99.18
FAD
enzyme contains 1.8 mol of FAD per mol of enzyme
Pseudomonas sp.
1.3.99.18
Pterin molybdenum cofactor
molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme
Pseudomonas sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.99.18
FAD
enzyme contains 1.8 mol of FAD per mol of enzyme
Pseudomonas sp.
1.3.99.18
Pterin molybdenum cofactor
molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.99.18
Acriflavine
-
Pseudomonas sp.
1.3.99.18
CoCl2
-
Pseudomonas sp.
1.3.99.18
Cu2+
-
Pseudomonas sp.
1.3.99.18
KCN
74% reactivation with S2-
Pseudomonas sp.
1.3.99.18
methanol
-
Pseudomonas sp.
1.3.99.18
Mn2+
-
Pseudomonas sp.
1.3.99.18
NaAsO2
-
Pseudomonas sp.
1.3.99.18
NiCl2
-
Pseudomonas sp.
1.3.99.18
p-hydroxymercuribenzoate
-
Pseudomonas sp.
1.3.99.18
Quinacrine
-
Pseudomonas sp.
1.3.99.18
ZnSO4
-
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.99.18
0.023
-
Quinaldate
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
Pseudomonas sp.
1.3.99.18
1.6
-
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
with quinaldate as cosubstrate
Pseudomonas sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.99.18
Iron
contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme
Pseudomonas sp.
1.3.99.18
Molybdenum
enzyme contains 1.6 g-atom per mol of enzyme
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.99.18
20000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
1.3.99.18
32000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
1.3.99.18
90000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
1.3.99.18
300000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.99.18
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.18
7.82
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.99.18
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
1.3.99.18
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
1.3.99.18
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
1.3.99.18
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
1.3.99.18
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
1.3.99.18
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
1.3.99.18
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp.
?
-
-
-
-
1.3.99.18
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp. AK-2
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.99.18
hexamer
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.99.18
60
-
above 60°C
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.99.18
75
-
5 min, 32% loss of activity
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.99.18
8
-
-
Pseudomonas sp.