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Literature summary extracted from

  • Peschke, B.; Lingens, F.
    Microbial metabolism of quinoline and related compounds. XII. Isolation and characterization of the quinoline oxidoreductase from Rhodococcus sp. B1 compared with the quinoline oxidoreductase from Pseudomonas putida 86 (1991), Biol. Chem. Hoppe-Seyler, 372, 1081-1088.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.17 4-hydroxymercuribenzoate
-
 Rhodococcus sp.
1.3.99.17 CN-
-
 Pseudomonas putida
1.3.99.17 CN-
-
 Rhodococcus sp.
1.3.99.17 methanol
-
 Pseudomonas putida
1.3.99.17 methanol
-
 Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.17 0.0025
-
 Quinoline
-
 Rhodococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.99.17 Fe contains 8 atoms of iron and 8 atoms of acid-labile sulfide Pseudomonas putida
1.3.99.17 Fe contains 8 atoms of iron and 8 atoms of acid-labile sulfide Rhodococcus sp.
1.3.99.17 Mo
-
 Pseudomonas putida
1.3.99.17 Mo the enzyme contains 1.3 mol of molybdenum per mol of enzyme Rhodococcus sp.
1.3.99.17 Mo enzyme contains 2 molecules of molybdopterin cytosine dinucleotide Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.17 18000
-
 x * 18000 + x * 32000 + x * 82000, SDS-PAGE Rhodococcus sp.
1.3.99.17 32000
-
 x * 18000 + x * 32000 + x * 82000, SDS-PAGE Rhodococcus sp.
1.3.99.17 82000
-
 x * 18000 + x * 32000 + x * 82000, SDS-PAGE Rhodococcus sp.
1.3.99.17 270000
-
 gel filtration Rhodococcus sp.
1.3.99.17 310000
-
 native PAGE Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.17 Pseudomonas putida
-
-
-
1.3.99.17 Pseudomonas putida 86
-
-
-
1.3.99.17 Rhodococcus sp.
-
-
-
1.3.99.17 Rhodococcus sp. B1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.17
-
 Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.17 5.8
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.17 (3-methyl)-quinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
 ?
1.3.99.17 (3-methyl)-quinoline + acceptor 20% of the activity with quinoline Pseudomonas putida (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
 ?
1.3.99.17 4-methylquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 4-methylquinoline + acceptor 20% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 6-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 6-hydroxyquinoline + acceptor 56% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 6-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 ?
-
 ?
1.3.99.17 6-hydroxyquinoline + acceptor 56% of the activity with quinoline Pseudomonas putida 86 ?
-
 ?
1.3.99.17 7-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 7-hydroxyquinoline + acceptor 27% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 7-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 ?
-
 ?
1.3.99.17 7-hydroxyquinoline + acceptor 27% of the activity with quinoline Pseudomonas putida 86 ?
-
 ?
1.3.99.17 8-chloroquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 8-chloroquinoline + acceptor 34% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 8-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 8-hydroxyquinoline + acceptor 25% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 8-methylquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 8-methylquinoline + acceptor acceptor: p-iodonitrotetrazolium violet Pseudomonas putida ?
-
 ?
1.3.99.17 8-methylquinoline + acceptor 22% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 quinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
1.3.99.17 quinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
1.3.99.17 quinoline + acceptor + H2O
-
 Rhodococcus sp. quinolin-2(1H)-one + reduced acceptor
-
 r
1.3.99.17 quinoline + acceptor + H2O
-
 Rhodococcus sp. B1 quinolin-2(1H)-one + reduced acceptor
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.99.17 ?
-
 Pseudomonas putida
1.3.99.17 ? x * 18000 + x * 32000 + x * 82000, SDS-PAGE Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.17 9.5
-
-
 Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.17 FAD enzyme contains 1.8 mol FAD per mol of enzyme Rhodococcus sp.
1.3.99.17 FAD enzyme contains 2 moleculs of FAD Pseudomonas putida
1.3.99.17 FAD enzyme contains 2 moleculs of FAD Rhodococcus sp.
1.3.99.17 molybdopterin
-
 Pseudomonas putida
1.3.99.17 molybdopterin enzyme contains 2 molecules of molybdopterin cytosine dinucleotide Rhodococcus sp.