BRENDA - Enzyme Database show

Purification and characterization of isoquinoline 1-oxidoreductase from Pseudomonas diminuata 7, a novel molybdenum-containing hydroxylase

Lehmann, M.; Tshisuaka, B.; Fetzner, S.; Roger, P.; Lingens, F.; J. Biol. Chem. 269, 11254-11260 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.99.16
1,10-phenanthroline
-
Brevundimonas diminuta
1.3.99.16
5-Hydroxyisoquinoline
rapid and very effective inactivation in presence as well as in absence of the electron acceptors iodonitrotetrazolium chloride, phenazine methosulfate or ferricyanide
Brevundimonas diminuta
1.3.99.16
arsenite
-
Brevundimonas diminuta
1.3.99.16
CN-
-
Brevundimonas diminuta
1.3.99.16
Isoquinoline
slow inhibition in absence of an electron acceptor
Brevundimonas diminuta
1.3.99.16
methanol
-
Brevundimonas diminuta
1.3.99.16
p-hydroxymercuribenzoate
-
Brevundimonas diminuta
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.99.16
Iron
enzyme contains 3.95 g-atom of iron per mol of enzyme. The iron and the acid-labile sulfur are arranged in two (2Fe-2S) clusters
Brevundimonas diminuta
1.3.99.16
Mo
enzyme contains 0.85 g-atom of molybdenum per mol of enzyme
Brevundimonas diminuta
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.99.16
16000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
1.3.99.16
80000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
1.3.99.16
93000
95000
gel filtration, analytical ultracentrifugation
Brevundimonas diminuta
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.99.16
Isoquinoline + acceptor + H2O
Brevundimonas diminuta
first enzyme of the isoquinoline catabolism
?
-
-
-
1.3.99.16
Isoquinoline + acceptor + H2O
Brevundimonas diminuta 7
first enzyme of the isoquinoline catabolism
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.99.16
Brevundimonas diminuta
-
-
-
1.3.99.16
Brevundimonas diminuta 7
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.99.16
-
Brevundimonas diminuta
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.16
13.87
-
-
Brevundimonas diminuta
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.99.16
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
1.3.99.16
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta 7
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
1.3.99.16
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
1.3.99.16
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta 7
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
1.3.99.16
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta
?
-
-
-
-
1.3.99.16
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta 7
?
-
-
-
-
1.3.99.16
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
1-oxophthalazine + reduced acceptor
-
-
-
-
1.3.99.16
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
1-oxophthalazine + reduced acceptor
-
-
-
-
1.3.99.16
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
1.3.99.16
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
Subunits
EC Number
Subunits
Commentary
Organism
1.3.99.16
dimer
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.99.16
55
60
-
Brevundimonas diminuta
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.99.16
60
-
5 min, rapid decrease of activity above
Brevundimonas diminuta
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.99.16
8.5
-
-
Brevundimonas diminuta
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.99.16
additional information
enzyme contains no FAD
Brevundimonas diminuta
1.3.99.16
Pterin molybdenum cofactor
-
Brevundimonas diminuta
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.99.16
additional information
enzyme contains no FAD
Brevundimonas diminuta
1.3.99.16
Pterin molybdenum cofactor
-
Brevundimonas diminuta
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.99.16
1,10-phenanthroline
-
Brevundimonas diminuta
1.3.99.16
5-Hydroxyisoquinoline
rapid and very effective inactivation in presence as well as in absence of the electron acceptors iodonitrotetrazolium chloride, phenazine methosulfate or ferricyanide
Brevundimonas diminuta
1.3.99.16
arsenite
-
Brevundimonas diminuta
1.3.99.16
CN-
-
Brevundimonas diminuta
1.3.99.16
Isoquinoline
slow inhibition in absence of an electron acceptor
Brevundimonas diminuta
1.3.99.16
methanol
-
Brevundimonas diminuta
1.3.99.16
p-hydroxymercuribenzoate
-
Brevundimonas diminuta
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.99.16
Iron
enzyme contains 3.95 g-atom of iron per mol of enzyme. The iron and the acid-labile sulfur are arranged in two (2Fe-2S) clusters
Brevundimonas diminuta
1.3.99.16
Mo
enzyme contains 0.85 g-atom of molybdenum per mol of enzyme
Brevundimonas diminuta
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.99.16
16000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
1.3.99.16
80000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
1.3.99.16
93000
95000
gel filtration, analytical ultracentrifugation
Brevundimonas diminuta
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.99.16
Isoquinoline + acceptor + H2O
Brevundimonas diminuta
first enzyme of the isoquinoline catabolism
?
-
-
-
1.3.99.16
Isoquinoline + acceptor + H2O
Brevundimonas diminuta 7
first enzyme of the isoquinoline catabolism
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.99.16
-
Brevundimonas diminuta
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.16
13.87
-
-
Brevundimonas diminuta
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.99.16
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
1.3.99.16
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta 7
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
1.3.99.16
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
1.3.99.16
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta 7
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
1.3.99.16
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta
?
-
-
-
-
1.3.99.16
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta 7
?
-
-
-
-
1.3.99.16
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
1-oxophthalazine + reduced acceptor
-
-
-
-
1.3.99.16
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
1-oxophthalazine + reduced acceptor
-
-
-
-
1.3.99.16
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
1.3.99.16
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.99.16
dimer
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.99.16
55
60
-
Brevundimonas diminuta
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.99.16
60
-
5 min, rapid decrease of activity above
Brevundimonas diminuta
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.99.16
8.5
-
-
Brevundimonas diminuta