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Literature summary extracted from

  • Park, I.S.; Walsh, C.T.
    D-Alanyl-D-lactate and D-alanyl-D-alanine synthesis by D-alanyl-D-alanine ligase from vancomycin-resistant Leuconostoc mesenteroides. Effects of a phenylalanine 261 to tyrosine mutation (1997), J. Biol. Chem., 272, 9210-9214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.4 overproduction in Escherichia coli, the vancomycin resistant enzyme has Phe261 instead of Tyr. The Tyr to Phe substitution on the active site omega-loop in the D-Ala-D-Ala ligases is a molecular indicator of both the ability to make D-Ala-D-Lac and intrinsic resistance to the vancomycin class of glycopeptide antibiotics Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.4 additional information
-
additional information
-
Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.4 Leuconostoc mesenteroides P71454 resistant to vancomycin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.4
-
Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.4 ATP + D-Ala + D-Ala
-
Leuconostoc mesenteroides ADP + phosphate + D-Ala-D-Ala
-
?
6.3.2.4 ATP + D-Ala + D-lactate
-
Leuconostoc mesenteroides ADP + phosphate + D-Ala-D-lactate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.4 additional information
-
additional information
-
Leuconostoc mesenteroides