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Literature summary extracted from

  • Vellekamp, G.J.; Coyle, C.L.; Kull, F.J.
    Low molecular weight aspartyl-tRNA synthetase from porcine thyroid. Purification, characterization, and heterogeneity (1983), J. Biol. Chem., 258, 8195-8200.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.12 Br-
-
 Sus scrofa
6.1.1.12 CaCl2 1 mM, more than 50% inhibition Sus scrofa
6.1.1.12 Cl-
-
 Sus scrofa
6.1.1.12 F-
-
 Sus scrofa
6.1.1.12 I-
-
 Sus scrofa
6.1.1.12 NiCl2 0.1 mM more than 50% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.12 0.000061
-
 tRNAAsp
-
 Sus scrofa
6.1.1.12 0.067
-
 Asp
-
 Sus scrofa
6.1.1.12 0.26
-
 ATP
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.12 Mg2+ required Sus scrofa
6.1.1.12 Mg2+ optimal Mg2+/ATP ratio is 2 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.12 53000
-
 2 * 53000, gel electrophoresis under denaturing conditions Sus scrofa
6.1.1.12 124000
-
 gel filtration Sus scrofa
6.1.1.12 156000
-
 nondenaturing PAGE Sus scrofa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
6.1.1.12 Glycerol 25% v/v, required throughout purification procedure to maintain stability Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12 enzyme form PC-1 (in addition 3 low MW enzyme forms exist: PC-2a, PC-2b and PC-2c) Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.12 thyroid gland
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
6.1.1.12 -20°C, 10 mM potassium phosphate, pH 7.6, 10 mM 2-mercaptoethanol, 50% glycerol, stable Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Sus scrofa AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.12 dimer 2 * 53000, gel electrophoresis under denaturing conditions Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.12 additional information
-
 additional information
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.12 7.8 8 potassium Hepes buffer Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.12 0.1
-
 NiCl2
-
 Sus scrofa
6.1.1.12 1
-
 CaCl2
-
 Sus scrofa