BRENDA - Enzyme Database show

Purification and characterization of a benzylviologen-linked tungsten-containing aldehyde oxidoreductase from Desulfovibrio gigas

Hensgens, C.M.H.; Hagen, W.R.; Hansen, T.A.; J. Bacteriol. 177, 6195-6200 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.99.6
AsO2-
-
Desulfovibrio gigas
1.2.99.6
CN-
-
Desulfovibrio gigas
1.2.99.6
iodoacetate
-
Desulfovibrio gigas
1.2.99.6
methanol
-
Desulfovibrio gigas
1.2.99.7
arsenite
5 mM, complete inhibition
Desulfovibrio gigas
1.2.99.7
iodoacetate
5 mM, complete inhibition
Desulfovibrio gigas
1.2.99.7
KCN
5 mM, 64% inhibition
Desulfovibrio gigas
1.2.99.7
methanol
1 M, 62% inhibition
Desulfovibrio gigas
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.99.6
0.0108
-
propanal
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.0125
-
acetaldehyde
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.02
-
benzaldehyde
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.0308
-
furfural
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.55
-
benzyl viologen
-
Desulfovibrio gigas
1.2.99.7
0.55
-
benzylviologen
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
10.8
-
propionaldehyde
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
12.5
-
acetaldehyde
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
20
-
benzaldehyde
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
30.8
-
furfural
30°C, pH 7.5
Desulfovibrio gigas
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.99.6
Iron
contains 4.8 gatom of Fe per mol of subunit; possibly contains a [4Fe-4S]1+cluster
Desulfovibrio gigas
1.2.99.6
K+
stimulates, optimal concentration: 10 mM
Desulfovibrio gigas
1.2.99.6
Tungsten
enzyme contains 0.68 gatom of tungsten per mol of subunit
Desulfovibrio gigas
1.2.99.7
Iron
4.8 iron per subunit, probably a [Fe-4S]1+ cluster
Desulfovibrio gigas
1.2.99.7
K+
10 mM, 50% activation
Desulfovibrio gigas
1.2.99.7
Tungsten
0.68 tungsten per subunit
Desulfovibrio gigas
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.99.6
62000
-
2 * 62000, SDS-PAGE
Desulfovibrio gigas
1.2.99.6
120000
126000
gel filtration
Desulfovibrio gigas
1.2.99.7
62000
-
2 * 62000, SDS-PAGE
Desulfovibrio gigas
1.2.99.7
120000
-
gelfiltration, Sephacryl S-300
Desulfovibrio gigas
1.2.99.7
126000
-
gel filtration, Superose 12 HR
Desulfovibrio gigas
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.99.6
Desulfovibrio gigas
-
-
-
1.2.99.6
Desulfovibrio gigas NCIMB 9332
-
-
-
1.2.99.7
Desulfovibrio gigas
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.2.99.6
unstable to air
Desulfovibrio gigas
1.2.99.7
exposure to air leads to inactivation
Desulfovibrio gigas
Purification (Commentary)
EC Number
Commentary
Organism
1.2.99.6
-
Desulfovibrio gigas
1.2.99.7
Q-Sepharose, Butyl-Sepharose, DEAE-memsep 1010
Desulfovibrio gigas
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.99.6
38.5
-
-
Desulfovibrio gigas
1.2.99.7
38.5
-
-
Desulfovibrio gigas
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.99.6
4-Methoxybenzaldehyde + benzyl viologen
at 19% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + 2,6-dichlorophenol-indophenol
at 1.3% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas
acetate + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
1.2.99.6
Acetaldehyde + 2,6-dichlorophenol-indophenol
at 1.3% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
1.2.99.6
Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
at 5% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas
acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
-
-
-
-
1.2.99.6
Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
at 5% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
-
11936
Desulfovibrio gigas
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
-
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + methyl viologen
at 19% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas
acetate + reduced methyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + methyl viologen
at 19% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced methyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
at 93% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
at 93% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas NCIMB 9332
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Butyraldehyde + benzyl viologen
at 16% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Butanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Furfural + benzyl viologen
at 77% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
2-Furancarboxylic acid + reduced benzyl viologen
-
-
-
-
1.2.99.6
Glutaraldehyde + benzyl viologen
at 16% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Glutarate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Glycolaldehyde + benzyl viologen
at 23% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Glycolate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Hexanal + benzyl viologen
at 6% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Hexanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Methanal + benzyl viologen
at 16% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Formate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Pentanal + benzyl viologen
at 9% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Pentanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
at 31% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Propanal + benzyl viologen
at 117% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Propanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Salicylaldehyde + benzyl viologen
at 14% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Salicylate + reduced benzyl viologen
-
-
-
-
1.2.99.7
acetaldehyde + H2O + benzylviologen
natural electron aceptor not yet known, the artificial electron acceptors benzylviologen, methylviologen, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl) and 2,6-dichlorphenol-indophenol have relative activities of 100%, 19%, 5%, and 1.3%, respectively
11936
Desulfovibrio gigas
acetate + reduced benzylviologen
-
-
-
?
1.2.99.7
benzaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
1.2.99.7
benzaldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
1.2.99.7
butyraldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
butanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
formaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
formate + reduced benzylviologen
-
-
-
?
1.2.99.7
furfural + H2O + benzylviologen
77% of activity with acetaldehyde
11936
Desulfovibrio gigas
furfurate + reduced benzylviologen
-
-
-
?
1.2.99.7
glutaraldehyde + H2O + benzylviologen
20% of activity with acetaldehyde
11936
Desulfovibrio gigas
glutarate + reduced benzylviologen
-
-
-
?
1.2.99.7
glycoaldehyde + H2O + benzylviologen
23% of activity with acetaldehyde
11936
Desulfovibrio gigas
glycolate + reduced benzylviologen
-
-
-
?
1.2.99.7
hexanal + H2O + benzylviologen
6% of activity with acetaldehyde
11936
Desulfovibrio gigas
hexanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
octanal + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
octanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
p-anisaldehyde + H2O + benzylviologen
5% of activity with acetaldehyde
11936
Desulfovibrio gigas
p-anisidic acid + reduced benzylviologen
-
-
-
?
1.2.99.7
pentanal + H2O + benzylviologen
9% of activity with acetaldehyde
11936
Desulfovibrio gigas
pentanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
phenylacetaldehyde + H2O + benzylviologen
31% of activity with acetaldehyde
11936
Desulfovibrio gigas
phenylacetate + reduced benzylviologen
-
-
-
?
1.2.99.7
propionaldehyde + H2O + benzylviologen
117% of activity with acetaldehyde
11936
Desulfovibrio gigas
propionate + reduced benzylviologen
-
-
-
?
1.2.99.7
salicylaldehyde + H2O + benzylviologen
14% of activity with acetaldehyde
11936
Desulfovibrio gigas
salicylate + reduced benzylviologen
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.99.6
dimer
2 * 62000, SDS-PAGE
Desulfovibrio gigas
1.2.99.7
dimer
2 * 62000, SDS-PAGE
Desulfovibrio gigas
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.99.6
48
-
and above
Desulfovibrio gigas
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.99.7
30
65
-
Desulfovibrio gigas
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.99.6
65
-
decrease of activity after 3 min
Desulfovibrio gigas
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.99.6
8
-
broad, with benzyl viologen and acetaldehyde as substrates
Desulfovibrio gigas
1.2.99.7
8
-
broad optimum
Desulfovibrio gigas
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.99.6
molybdopterin
enzyme contains molybdopterin
Desulfovibrio gigas
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.99.6
molybdopterin
enzyme contains molybdopterin
Desulfovibrio gigas
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.99.6
AsO2-
-
Desulfovibrio gigas
1.2.99.6
CN-
-
Desulfovibrio gigas
1.2.99.6
iodoacetate
-
Desulfovibrio gigas
1.2.99.6
methanol
-
Desulfovibrio gigas
1.2.99.7
arsenite
5 mM, complete inhibition
Desulfovibrio gigas
1.2.99.7
iodoacetate
5 mM, complete inhibition
Desulfovibrio gigas
1.2.99.7
KCN
5 mM, 64% inhibition
Desulfovibrio gigas
1.2.99.7
methanol
1 M, 62% inhibition
Desulfovibrio gigas
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.99.6
0.0108
-
propanal
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.0125
-
acetaldehyde
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.02
-
benzaldehyde
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.0308
-
furfural
with benzyl viologen as cosubstrate
Desulfovibrio gigas
1.2.99.6
0.55
-
benzyl viologen
-
Desulfovibrio gigas
1.2.99.7
0.55
-
benzylviologen
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
10.8
-
propionaldehyde
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
12.5
-
acetaldehyde
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
20
-
benzaldehyde
30°C, pH 7.5
Desulfovibrio gigas
1.2.99.7
30.8
-
furfural
30°C, pH 7.5
Desulfovibrio gigas
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.99.6
Iron
contains 4.8 gatom of Fe per mol of subunit; possibly contains a [4Fe-4S]1+cluster
Desulfovibrio gigas
1.2.99.6
K+
stimulates, optimal concentration: 10 mM
Desulfovibrio gigas
1.2.99.6
Tungsten
enzyme contains 0.68 gatom of tungsten per mol of subunit
Desulfovibrio gigas
1.2.99.7
Iron
4.8 iron per subunit, probably a [Fe-4S]1+ cluster
Desulfovibrio gigas
1.2.99.7
K+
10 mM, 50% activation
Desulfovibrio gigas
1.2.99.7
Tungsten
0.68 tungsten per subunit
Desulfovibrio gigas
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.99.6
62000
-
2 * 62000, SDS-PAGE
Desulfovibrio gigas
1.2.99.6
120000
126000
gel filtration
Desulfovibrio gigas
1.2.99.7
62000
-
2 * 62000, SDS-PAGE
Desulfovibrio gigas
1.2.99.7
120000
-
gelfiltration, Sephacryl S-300
Desulfovibrio gigas
1.2.99.7
126000
-
gel filtration, Superose 12 HR
Desulfovibrio gigas
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.2.99.6
unstable to air
Desulfovibrio gigas
1.2.99.7
exposure to air leads to inactivation
Desulfovibrio gigas
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.99.6
-
Desulfovibrio gigas
1.2.99.7
Q-Sepharose, Butyl-Sepharose, DEAE-memsep 1010
Desulfovibrio gigas
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.99.6
38.5
-
-
Desulfovibrio gigas
1.2.99.7
38.5
-
-
Desulfovibrio gigas
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.99.6
4-Methoxybenzaldehyde + benzyl viologen
at 19% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + 2,6-dichlorophenol-indophenol
at 1.3% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas
acetate + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
1.2.99.6
Acetaldehyde + 2,6-dichlorophenol-indophenol
at 1.3% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
1.2.99.6
Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
at 5% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas
acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
-
-
-
-
1.2.99.6
Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
at 5% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
-
11936
Desulfovibrio gigas
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
-
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + methyl viologen
at 19% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas
acetate + reduced methyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + methyl viologen
at 19% of the activity relative to benzyl viologen
11936
Desulfovibrio gigas NCIMB 9332
acetate + reduced methyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
at 93% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
at 93% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas NCIMB 9332
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Butyraldehyde + benzyl viologen
at 16% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Butanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Furfural + benzyl viologen
at 77% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
2-Furancarboxylic acid + reduced benzyl viologen
-
-
-
-
1.2.99.6
Glutaraldehyde + benzyl viologen
at 16% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Glutarate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Glycolaldehyde + benzyl viologen
at 23% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Glycolate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Hexanal + benzyl viologen
at 6% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Hexanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Methanal + benzyl viologen
at 16% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Formate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Pentanal + benzyl viologen
at 9% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Pentanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
at 31% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Propanal + benzyl viologen
at 117% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Propanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Salicylaldehyde + benzyl viologen
at 14% of the activity relative to acetaldehyde
11936
Desulfovibrio gigas
Salicylate + reduced benzyl viologen
-
-
-
-
1.2.99.7
acetaldehyde + H2O + benzylviologen
natural electron aceptor not yet known, the artificial electron acceptors benzylviologen, methylviologen, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl) and 2,6-dichlorphenol-indophenol have relative activities of 100%, 19%, 5%, and 1.3%, respectively
11936
Desulfovibrio gigas
acetate + reduced benzylviologen
-
-
-
?
1.2.99.7
benzaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
1.2.99.7
benzaldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
1.2.99.7
butyraldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
butanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
formaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
formate + reduced benzylviologen
-
-
-
?
1.2.99.7
furfural + H2O + benzylviologen
77% of activity with acetaldehyde
11936
Desulfovibrio gigas
furfurate + reduced benzylviologen
-
-
-
?
1.2.99.7
glutaraldehyde + H2O + benzylviologen
20% of activity with acetaldehyde
11936
Desulfovibrio gigas
glutarate + reduced benzylviologen
-
-
-
?
1.2.99.7
glycoaldehyde + H2O + benzylviologen
23% of activity with acetaldehyde
11936
Desulfovibrio gigas
glycolate + reduced benzylviologen
-
-
-
?
1.2.99.7
hexanal + H2O + benzylviologen
6% of activity with acetaldehyde
11936
Desulfovibrio gigas
hexanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
octanal + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
octanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
p-anisaldehyde + H2O + benzylviologen
5% of activity with acetaldehyde
11936
Desulfovibrio gigas
p-anisidic acid + reduced benzylviologen
-
-
-
?
1.2.99.7
pentanal + H2O + benzylviologen
9% of activity with acetaldehyde
11936
Desulfovibrio gigas
pentanoate + reduced benzylviologen
-
-
-
?
1.2.99.7
phenylacetaldehyde + H2O + benzylviologen
31% of activity with acetaldehyde
11936
Desulfovibrio gigas
phenylacetate + reduced benzylviologen
-
-
-
?
1.2.99.7
propionaldehyde + H2O + benzylviologen
117% of activity with acetaldehyde
11936
Desulfovibrio gigas
propionate + reduced benzylviologen
-
-
-
?
1.2.99.7
salicylaldehyde + H2O + benzylviologen
14% of activity with acetaldehyde
11936
Desulfovibrio gigas
salicylate + reduced benzylviologen
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.99.6
dimer
2 * 62000, SDS-PAGE
Desulfovibrio gigas
1.2.99.7
dimer
2 * 62000, SDS-PAGE
Desulfovibrio gigas
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.99.6
48
-
and above
Desulfovibrio gigas
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.99.7
30
65
-
Desulfovibrio gigas
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.99.6
65
-
decrease of activity after 3 min
Desulfovibrio gigas
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.99.6
8
-
broad, with benzyl viologen and acetaldehyde as substrates
Desulfovibrio gigas
1.2.99.7
8
-
broad optimum
Desulfovibrio gigas