BRENDA - Enzyme Database show

Purification, characterization, and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic and proteolytic archaeon Thermococcus strain ES-1

Heider, J.; Ma, K.; Adams, M.W.W.; J. Bacteriol. 177, 4757-4764 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.5
2,2'-bipyridyl
competitive to methyl viologen
Thermococcus sp.
1.2.7.5
acetaldehyde
50% inhibition at 5 mM
Thermococcus sp.
1.2.7.5
aldehydes
substrate inhibition at high concentrations, overview
Thermococcus sp.
1.2.7.5
arsenite
50% inhibition at 0.5 mM
Thermococcus sp.
1.2.7.5
crotonaldehyde
50% inhibition at 20 mM
Thermococcus sp.
1.2.7.5
iodoacetate
50% inhibition at 0.5 mM
Thermococcus sp.
1.2.7.5
isovalerylaldehyde
50% inhibition at 4 mM
Thermococcus sp.
1.2.7.5
p-chloromercuribenzoate
-
Thermococcus sp.
1.2.7.5
phenylacetaldehyde
50% inhibition at 7 mM
Thermococcus sp.
1.2.7.5
propionaldehyde
50% inhibition at 0.6 mM
Thermococcus sp.
1.2.7.5
Zn2+
50% inhibition at 0.25 mM
Thermococcus sp.
1.2.99.6
2,2'-bipyridyl
-
Thermococcus sp.
1.2.99.6
acetaldehyde
5 mM, 50% inhibition
Thermococcus sp.
1.2.99.6
AsO2-
-
Thermococcus sp.
1.2.99.6
CN-
relatively insensitive, 10% inhibition after 1 with 8 mM
Thermococcus sp.
1.2.99.6
iodoacetate
-
Thermococcus sp.
1.2.99.6
Isovaleraldehyde
4 mM, 50% inhibition
Thermococcus sp.
1.2.99.6
PCMB
-
Thermococcus sp.
1.2.99.6
phenylacetaldehyde
7 mM, 50% inhibition
Thermococcus sp.
1.2.99.6
ZnCl2
-
Thermococcus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.5
0.01
-
Ferredoxin
ES-1 ferredoxin; pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
0.016
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.028
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.05
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.057
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.065
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.076
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.13
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.14
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.15
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.17
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
0.2
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
1.42
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
1.8
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
2.9
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.99.6
0.01
-
ES-1 ferredoxin
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.016
-
acetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.028
-
Isovaleraldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.05
-
Indoleacetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.057
-
benzaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.065
-
Salicylaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.076
-
phenylacetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.13
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
0.136
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.15
-
propanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.169
-
benzyl viologen
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.196
-
glyceraldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
1.422
-
methanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
1.8
-
acetate
with methylviologen as cosubstrate
Thermococcus sp.
1.2.99.6
2.9
-
methyl viologen
with crotonaldehyde as cosubstrate
Thermococcus sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.5
Ca2+
0.7 gatoms per subunit
Thermococcus sp.
1.2.7.5
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit
Thermococcus sp.
1.2.7.5
Mg2+
contains 1 Mg-atom per subunit
Thermococcus sp.
1.2.7.5
Molybdenum
-
Thermococcus sp.
1.2.7.5
Tungsten
1 tungsten per subunit
Thermococcus sp.
1.2.7.5
Zn2+
0.2 gatoms per subunit
Thermococcus sp.
1.2.99.6
Ca2+
the enzyme contains 0.7 gatom of Ca per mol of enzyme
Thermococcus sp.
1.2.99.6
Iron
contains a single [4Fe-4S]+cluster with an S = 3/2 ground state; enzyme contains 3.6 gatom Fe per mol of enzyme
Thermococcus sp.
1.2.99.6
Mg2+
enzyme contains 1.1 gatom of Mg per mol of enzyme
Thermococcus sp.
1.2.99.6
Tungsten
enzyme contains 0.9 gatom of tungsten per mol of enzyme
Thermococcus sp.
1.2.99.6
Zn2+
the enzyme contains 0.2 gatom of Zn per mol of enzyme
Thermococcus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.5
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
1.2.7.5
105000
-
gel filtration
Thermococcus sp.
1.2.99.6
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
1.2.99.6
105000
-
gel filtration
Thermococcus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp.
enzyme oxidizes aldehydes generated during amino acid catabolism
an acid + H+ + reduced ferredoxin
-
-
?
1.2.99.6
additional information
Thermococcus sp.
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
?
-
-
-
1.2.99.6
additional information
Thermococcus sp. ES-1
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
?
-
-
-
1.2.99.6
additional information
Thermococcus sp. ES1
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.5
Thermococcus sp.
-
hyperthermophilic archaeon
-
1.2.7.5
Thermococcus sp. ES1
-
hyperthermophilic archaeon
-
1.2.99.6
Thermococcus sp.
-
-
-
1.2.99.6
Thermococcus sp. ES-1
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.2.7.5
very sensitive to O2, after 1 h at 60C 3% activity remains, after 1 h at 4C 39% activity remains
Thermococcus sp.
1.2.99.6
extremely sensitive to inactivation by O2. The extent of inactivation is greatly dependent on the temperature of incubation
Thermococcus sp.
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.5
about 120fold, to homogeneity
Thermococcus sp.
1.2.99.6
-
Thermococcus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.99.6
culture condition:ethanol-grown cell
-
Thermococcus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.7.5
238
-
purified enzyme
Thermococcus sp.
1.2.99.6
221
-
-
Thermococcus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.5
acetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
acetate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
acetaldehyde + H2O + oxidized ferredoxin
best substrate
11935
Thermococcus sp.
acetate + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
1.2.7.5
acetate + H+ + reduced methyl viologen
very low activity
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
acetate + H+ + reduced methyl viologen
below pH 6.0
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
-
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
enzyme oxidizes aldehydes generated during amino acid catabolism
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
-
-
?
1.2.7.5
benzaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
benzoic acid + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
crotonaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
formaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
formate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
glyceraldehyde + H2O + oxidized methyl viologen
very poor substrate
11935
Thermococcus sp.
glycerate + H+ + reduced methyl viologen
-
-
-
-
1.2.7.5
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
isovalerate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
additional information
acid reduction is strongly dependent on the overall reduction potential, therfore reverse reaction is only possible with reduced methyl viologen at a low rate
11935
Thermococcus sp.
?
-
-
-
-
1.2.7.5
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
phenylacetate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
propionaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
propionate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
salicylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
salicylic acid + H+ + reduced benzyl viologen
-
-
-
ir
1.2.99.6
Acetaldehyde + benzyl viologen
r
11935
Thermococcus sp.
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
r
11935
Thermococcus sp. ES-1
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
r
11935
Thermococcus sp. ES1
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES-1
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES1
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Crotonaldehyde + benzyl viologen
at less than 1% of the activity with benzyl viologen
11935
Thermococcus sp.
E-2-Butenoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Crotonaldehyde + ES-1 ferredoxin
-
11935
Thermococcus sp.
Crotonate + reduced ES-1 ferredoxin
-
-
-
-
1.2.99.6
Crotonaldehyde + methyl viologen
-
11935
Thermococcus sp.
Crotonate + reduced methyl viologen
-
-
-
-
1.2.99.6
Glyceraldehyde + benzyl viologen
-
11935
Thermococcus sp.
Glycerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Indoleacetaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Indoleacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Isovaleraldehyde + benzyl viologen
-
11935
Thermococcus sp.
Isovalerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Isovaleraldehyde + benzyl viologen
-
11935
Thermococcus sp. ES-1
Isovalerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Isovaleraldehyde + benzyl viologen
-
11935
Thermococcus sp. ES1
Isovalerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Methanal + benzyl viologen
-
11935
Thermococcus sp.
Formate + reduced benzyl viologen
-
-
-
-
1.2.99.6
additional information
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
11935
Thermococcus sp.
?
-
-
-
-
1.2.99.6
additional information
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
11935
Thermococcus sp. ES-1
?
-
-
-
-
1.2.99.6
additional information
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
11935
Thermococcus sp. ES1
?
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES-1
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES1
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Propanal + benzyl viologen
-
11935
Thermococcus sp.
Propanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Salicylaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Salicylate + reduced benzyl viologen
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.5
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
1.2.99.6
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.7.5
85
-
assay at
Thermococcus sp.
1.2.99.6
85
-
-
Thermococcus sp.
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.7.5
additional information
-
the enzyme is thermostable
Thermococcus sp.
1.2.7.5
23
-
pure enzyme, 2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, irreversible loss of 50% activity after 1 min
Thermococcus sp.
1.2.99.6
70
-
enzyme concentration 2.0 mg/ml, 50% loss of activity after 5 min
Thermococcus sp.
1.2.99.6
85
-
enzyme concentration 2.0 mg/ml, 50% loss of activity after 1 min
Thermococcus sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.7.5
1.6
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
2.8
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
13
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
22
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
55
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
62
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
250
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
269
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
270
-
Ferredoxin
ES-1 ferredoxin, pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
272
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
343
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
720
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
950
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
960
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
1100
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.99.6
1.6
-
acetaldehyde
with methyl viologen as cosubstrate
Thermococcus sp.
1.2.99.6
10.5
-
glyceraldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
13
-
Salicylaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
22
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
55
-
indolecetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
62
-
methyl viologen
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
250
-
benzyl viologen
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
269
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
270
-
ES-1 ferredoxin
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
272
-
Isovaleraldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
343
-
acetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
720
-
benzaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
950
-
methanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
960
-
phenylacetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
1100
-
propanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.5
8.4
-
assay at
Thermococcus sp.
1.2.99.6
8
-
around, at 80 C, with benzyl viologen and crotonaldehyde as substrate
Thermococcus sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.7.5
6
10
oxidation of acetaldehyde with benzyl viologen as electron acceptor
Thermococcus sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.7.5
Ferredoxin
-
Thermococcus sp.
1.2.7.5
form A cofactor
in presence of iodine
Thermococcus sp.
1.2.7.5
form B cofactor
in absence of iodine
Thermococcus sp.
1.2.7.5
molybdopterin
-
Thermococcus sp.
1.2.99.6
molybdopterin
enzyme contains molybdopterin
Thermococcus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.7.5
Ferredoxin
-
Thermococcus sp.
1.2.7.5
form A cofactor
in presence of iodine
Thermococcus sp.
1.2.7.5
form B cofactor
in absence of iodine
Thermococcus sp.
1.2.7.5
molybdopterin
-
Thermococcus sp.
1.2.99.6
molybdopterin
enzyme contains molybdopterin
Thermococcus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.5
2,2'-bipyridyl
competitive to methyl viologen
Thermococcus sp.
1.2.7.5
acetaldehyde
50% inhibition at 5 mM
Thermococcus sp.
1.2.7.5
aldehydes
substrate inhibition at high concentrations, overview
Thermococcus sp.
1.2.7.5
arsenite
50% inhibition at 0.5 mM
Thermococcus sp.
1.2.7.5
crotonaldehyde
50% inhibition at 20 mM
Thermococcus sp.
1.2.7.5
iodoacetate
50% inhibition at 0.5 mM
Thermococcus sp.
1.2.7.5
isovalerylaldehyde
50% inhibition at 4 mM
Thermococcus sp.
1.2.7.5
p-chloromercuribenzoate
-
Thermococcus sp.
1.2.7.5
phenylacetaldehyde
50% inhibition at 7 mM
Thermococcus sp.
1.2.7.5
propionaldehyde
50% inhibition at 0.6 mM
Thermococcus sp.
1.2.7.5
Zn2+
50% inhibition at 0.25 mM
Thermococcus sp.
1.2.99.6
2,2'-bipyridyl
-
Thermococcus sp.
1.2.99.6
acetaldehyde
5 mM, 50% inhibition
Thermococcus sp.
1.2.99.6
AsO2-
-
Thermococcus sp.
1.2.99.6
CN-
relatively insensitive, 10% inhibition after 1 with 8 mM
Thermococcus sp.
1.2.99.6
iodoacetate
-
Thermococcus sp.
1.2.99.6
Isovaleraldehyde
4 mM, 50% inhibition
Thermococcus sp.
1.2.99.6
PCMB
-
Thermococcus sp.
1.2.99.6
phenylacetaldehyde
7 mM, 50% inhibition
Thermococcus sp.
1.2.99.6
ZnCl2
-
Thermococcus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.5
0.01
-
Ferredoxin
ES-1 ferredoxin; pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
0.016
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.028
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.05
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.057
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.065
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.076
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.13
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.14
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.15
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
0.17
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
0.2
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
1.42
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
1.8
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
2.9
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.99.6
0.01
-
ES-1 ferredoxin
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.016
-
acetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.028
-
Isovaleraldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.05
-
Indoleacetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.057
-
benzaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.065
-
Salicylaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.076
-
phenylacetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.13
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
0.136
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.15
-
propanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.169
-
benzyl viologen
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
0.196
-
glyceraldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
1.422
-
methanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
1.8
-
acetate
with methylviologen as cosubstrate
Thermococcus sp.
1.2.99.6
2.9
-
methyl viologen
with crotonaldehyde as cosubstrate
Thermococcus sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.5
Ca2+
0.7 gatoms per subunit
Thermococcus sp.
1.2.7.5
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit
Thermococcus sp.
1.2.7.5
Mg2+
contains 1 Mg-atom per subunit
Thermococcus sp.
1.2.7.5
Molybdenum
-
Thermococcus sp.
1.2.7.5
Tungsten
1 tungsten per subunit
Thermococcus sp.
1.2.7.5
Zn2+
0.2 gatoms per subunit
Thermococcus sp.
1.2.99.6
Ca2+
the enzyme contains 0.7 gatom of Ca per mol of enzyme
Thermococcus sp.
1.2.99.6
Iron
contains a single [4Fe-4S]+cluster with an S = 3/2 ground state; enzyme contains 3.6 gatom Fe per mol of enzyme
Thermococcus sp.
1.2.99.6
Mg2+
enzyme contains 1.1 gatom of Mg per mol of enzyme
Thermococcus sp.
1.2.99.6
Tungsten
enzyme contains 0.9 gatom of tungsten per mol of enzyme
Thermococcus sp.
1.2.99.6
Zn2+
the enzyme contains 0.2 gatom of Zn per mol of enzyme
Thermococcus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.5
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
1.2.7.5
105000
-
gel filtration
Thermococcus sp.
1.2.99.6
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
1.2.99.6
105000
-
gel filtration
Thermococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp.
enzyme oxidizes aldehydes generated during amino acid catabolism
an acid + H+ + reduced ferredoxin
-
-
?
1.2.99.6
additional information
Thermococcus sp.
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
?
-
-
-
1.2.99.6
additional information
Thermococcus sp. ES-1
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
?
-
-
-
1.2.99.6
additional information
Thermococcus sp. ES1
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
?
-
-
-
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.2.7.5
very sensitive to O2, after 1 h at 60C 3% activity remains, after 1 h at 4C 39% activity remains
Thermococcus sp.
1.2.99.6
extremely sensitive to inactivation by O2. The extent of inactivation is greatly dependent on the temperature of incubation
Thermococcus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.5
about 120fold, to homogeneity
Thermococcus sp.
1.2.99.6
-
Thermococcus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.99.6
culture condition:ethanol-grown cell
-
Thermococcus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.7.5
238
-
purified enzyme
Thermococcus sp.
1.2.99.6
221
-
-
Thermococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.5
acetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
acetate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
acetaldehyde + H2O + oxidized ferredoxin
best substrate
11935
Thermococcus sp.
acetate + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
1.2.7.5
acetate + H+ + reduced methyl viologen
very low activity
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
acetate + H+ + reduced methyl viologen
below pH 6.0
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
-
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
enzyme oxidizes aldehydes generated during amino acid catabolism
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
-
-
?
1.2.7.5
benzaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
benzoic acid + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
crotonaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
formaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
formate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
glyceraldehyde + H2O + oxidized methyl viologen
very poor substrate
11935
Thermococcus sp.
glycerate + H+ + reduced methyl viologen
-
-
-
-
1.2.7.5
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
isovalerate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
additional information
acid reduction is strongly dependent on the overall reduction potential, therfore reverse reaction is only possible with reduced methyl viologen at a low rate
11935
Thermococcus sp.
?
-
-
-
-
1.2.7.5
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
phenylacetate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
propionaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
propionate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
1.2.7.5
salicylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
salicylic acid + H+ + reduced benzyl viologen
-
-
-
ir
1.2.99.6
Acetaldehyde + benzyl viologen
r
11935
Thermococcus sp.
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
r
11935
Thermococcus sp. ES-1
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Acetaldehyde + benzyl viologen
r
11935
Thermococcus sp. ES1
acetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES-1
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Benzaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES1
Benzoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Crotonaldehyde + benzyl viologen
at less than 1% of the activity with benzyl viologen
11935
Thermococcus sp.
E-2-Butenoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Crotonaldehyde + ES-1 ferredoxin
-
11935
Thermococcus sp.
Crotonate + reduced ES-1 ferredoxin
-
-
-
-
1.2.99.6
Crotonaldehyde + methyl viologen
-
11935
Thermococcus sp.
Crotonate + reduced methyl viologen
-
-
-
-
1.2.99.6
Glyceraldehyde + benzyl viologen
-
11935
Thermococcus sp.
Glycerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Indoleacetaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Indoleacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Isovaleraldehyde + benzyl viologen
-
11935
Thermococcus sp.
Isovalerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Isovaleraldehyde + benzyl viologen
-
11935
Thermococcus sp. ES-1
Isovalerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Isovaleraldehyde + benzyl viologen
-
11935
Thermococcus sp. ES1
Isovalerate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Methanal + benzyl viologen
-
11935
Thermococcus sp.
Formate + reduced benzyl viologen
-
-
-
-
1.2.99.6
additional information
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
11935
Thermococcus sp.
?
-
-
-
-
1.2.99.6
additional information
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
11935
Thermococcus sp. ES-1
?
-
-
-
-
1.2.99.6
additional information
the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation
11935
Thermococcus sp. ES1
?
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES-1
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Phenylacetaldehyde + benzyl viologen
-
11935
Thermococcus sp. ES1
Phenylacetate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Propanal + benzyl viologen
-
11935
Thermococcus sp.
Propanoate + reduced benzyl viologen
-
-
-
-
1.2.99.6
Salicylaldehyde + benzyl viologen
-
11935
Thermococcus sp.
Salicylate + reduced benzyl viologen
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.5
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
1.2.99.6
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.7.5
85
-
assay at
Thermococcus sp.
1.2.99.6
85
-
-
Thermococcus sp.
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.7.5
additional information
-
the enzyme is thermostable
Thermococcus sp.
1.2.7.5
23
-
pure enzyme, 2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, irreversible loss of 50% activity after 1 min
Thermococcus sp.
1.2.99.6
70
-
enzyme concentration 2.0 mg/ml, 50% loss of activity after 5 min
Thermococcus sp.
1.2.99.6
85
-
enzyme concentration 2.0 mg/ml, 50% loss of activity after 1 min
Thermococcus sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.7.5
1.6
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
2.8
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
13
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
22
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
55
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
62
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
250
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
269
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
270
-
Ferredoxin
ES-1 ferredoxin, pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
1.2.7.5
272
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
343
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
720
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
950
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
960
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.7.5
1100
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.2.99.6
1.6
-
acetaldehyde
with methyl viologen as cosubstrate
Thermococcus sp.
1.2.99.6
10.5
-
glyceraldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
13
-
Salicylaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
22
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 45C
Thermococcus sp.
1.2.99.6
55
-
indolecetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
62
-
methyl viologen
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
250
-
benzyl viologen
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
269
-
crotonaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
270
-
ES-1 ferredoxin
with crotonaldehyde as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
272
-
Isovaleraldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
343
-
acetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
720
-
benzaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
950
-
methanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
960
-
phenylacetaldehyde
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
1.2.99.6
1100
-
propanal
with benzyl viologen as cosubstrate, at 85C
Thermococcus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.5
8.4
-
assay at
Thermococcus sp.
1.2.99.6
8
-
around, at 80 C, with benzyl viologen and crotonaldehyde as substrate
Thermococcus sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.7.5
6
10
oxidation of acetaldehyde with benzyl viologen as electron acceptor
Thermococcus sp.