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Literature summary extracted from
- A molybdenum and a tungsten isoenzyme of formylmethanofuran dehydrogenase in the thermophilic archaeon Methanobacterium wolfei (1992), Eur. J. Biochem., 209, 1013-1018.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.12 | CN- | molybdenum-containing isoenzyme | Methanothermobacter wolfeii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.7.12 | 0.013 | - |
N-formylmethanofuran | molybdenum enzyme | Methanothermobacter wolfeii | |
| 1.2.7.12 | 0.035 | - |
formate | - |
Methanothermobacter wolfeii | |
| 1.2.7.12 | 0.037 | - |
methylviologen | molybdenum enzyme | Methanothermobacter wolfeii | |
| 1.2.7.12 | 0.053 | - |
N-Furfurylformamide | molybdenum enzyme | Methanothermobacter wolfeii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.12 | Iron | the molybdenum isoenzyme contains 6.6 mol non-heme iron per mol of the 146000 MW protein | Methanothermobacter wolfeii | |
| 1.2.7.12 | Molybdenum | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter wolfeii | |
| 1.2.7.12 | Molybdenum | enzyme exists as a molybdenum containing isoenzyme and as a tungsten containing isoenzyme | Methanothermobacter wolfeii | |
| 1.2.7.12 | Molybdenum | molybdenum isoenzyme contains approximately 0.23 mol molybdenum and 0.46 mol molybdopterin guanine dinucleotide per mol of the 146000 MW protein | Methanothermobacter wolfeii | |
| 1.2.7.12 | Tungsten | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter wolfeii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.12 | 31000 | - |
or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | 31000 | - |
or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | 51000 | - |
or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | 51000 | - |
or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | 64000 | - |
or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | 64000 | - |
or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | 130000 | - |
native PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | 260000 | - |
gel filtration | Methanothermobacter wolfeii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.7.12 | Methanothermobacter wolfeii | - |
a molybdenum-containing isoenzyme and a tungsten-containing isoenzyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.7.12 | molybdenum-containing isoenzyme | Methanothermobacter wolfeii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.12 | 27.3 | - |
- |
Methanothermobacter wolfeii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.12 | Formate + oxidized acceptor | - |
Methanothermobacter wolfeii | ? | - |
? | |
| 1.2.7.12 | formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter wolfeii | methanofuran + reduced methylviologen + CO2 | - |
? | |
| 1.2.7.12 | N-Furfurylformamide + oxidized acceptor | - |
Methanothermobacter wolfeii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.7.12 | hexamer | or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| 1.2.7.12 | trimer | or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.12 | 55 | - |
pH 7.4, thermostable up to | Methanothermobacter wolfeii |
| 1.2.7.12 | 65 | - |
pH 7.4, in presence of 0.1 M ammonium sulfate, 0.1 M potassium sulfate, and 0.5 M potassium chloride, thermostable up to | Methanothermobacter wolfeii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.12 | 7.5 | - |
maximal activity above | Methanothermobacter wolfeii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.12 | 6.5 | 7.5 | no activity below pH 6.5, maximal activity above pH 7.5 | Methanothermobacter wolfeii |
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