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Literature summary extracted from

  • Dennda, G.; Kula, M.R.
    Purification and evaluation of the glutathione-synthesizing enzymes from Candida boidinii for cell-free synthesis of glutathione (1986), J. Biotechnol., 4, 143-158.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 Ca2+ 2 mM, strong [Candida] boidinii
6.3.2.3 CN-
-
 [Candida] boidinii
6.3.2.3 Fe2+ 2 mM, strong [Candida] boidinii
6.3.2.3 GSSG no inhibition by reduced glutathione [Candida] boidinii
6.3.2.3 iodoacetate
-
 [Candida] boidinii
6.3.2.3 NEM
-
 [Candida] boidinii
6.3.2.3 PCMB
-
 [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 0.3
-
 gamma-Glu-Cys
-
 [Candida] boidinii
6.3.2.3 0.48
-
 ATP
-
 [Candida] boidinii
6.3.2.3 0.8
-
 Gly
-
 [Candida] boidinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.3 K+ 5fold stimulation at 40 mM [Candida] boidinii
6.3.2.3 Mg2+ activates [Candida] boidinii
6.3.2.3 Mn2+ 40% of the activation rate of Mg2+, in the optimal range: 2-4 mM [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.3 59000
-
 2 * 59000, SDS-PAGE [Candida] boidinii
6.3.2.3 120000
-
 gel filtration [Candida] boidinii

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Brettanomyces abstinens
-
-
-
6.3.2.3 Cyberlindnera jadinii
-
-
-
6.3.2.3 Ogataea angusta
-
-
-
6.3.2.3 Pichia kudriavzevii
-
-
-
6.3.2.3 Pichia membranifaciens
-
-
-
6.3.2.3 Saccharomyces cerevisiae
-
-
-
6.3.2.3 [Candida] boidinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3
-
 [Candida] boidinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 0.17
-
-
 [Candida] boidinii

Storage Stability

EC Number Storage Stability Organism
6.3.2.3 -18°C, stable for at least 1 year [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Cyberlindnera jadinii ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Saccharomyces cerevisiae ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Ogataea angusta ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Pichia membranifaciens ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Brettanomyces abstinens ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Pichia kudriavzevii ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 [Candida] boidinii ADP + phosphate + glutathione i.e. gamma-Glu-Cys-Gly ?

Subunits

EC Number Subunits Comment Organism
6.3.2.3 dimer 2 * 59000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.3 37
-
-
 [Candida] boidinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.3 50
-
 pH 8.3, 40% loss of activity after 1 h in 0.15 M Tris-HCl buffer [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 7 8.5
-
 [Candida] boidinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.2.3 7 9 maximal stability after 24 h [Candida] boidinii