BRENDA - Enzyme Database show

Isolation and characterization of intracellular phytase from Macrophormina physeolina

Ghareib, M.; Youssef, K.A.; Nour el Dein, M.M.; Zentralbl. Mikrobiol. 143, 397-403 (1988)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.8
2,4-dinitrophenol
-
Macrophomina phaseolina
3.1.3.8
arsenate
-
Macrophomina phaseolina
3.1.3.8
arsenite
-
Macrophomina phaseolina
3.1.3.8
azide
-
Macrophomina phaseolina
3.1.3.8
EDTA
-
Macrophomina phaseolina
3.1.3.8
F-
-
Macrophomina phaseolina
3.1.3.8
Hg2+
-
Macrophomina phaseolina
3.1.3.8
iodoacetic acid
-
Macrophomina phaseolina
3.1.3.8
phloridzin
-
Macrophomina phaseolina
3.1.3.8
Tartrate
-
Macrophomina phaseolina
3.1.3.8
Zn2+
-
Macrophomina phaseolina
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.8
0.91
-
myo-inositol hexakisphosphate
-
Macrophomina phaseolina
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.8
Ca2+
activates
Macrophomina phaseolina
3.1.3.8
K+
activates
Macrophomina phaseolina
3.1.3.8
Zn2+
activates
Macrophomina phaseolina
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.3.8
additional information
high resistance to the denaturing effect of organic solvents
Macrophomina phaseolina
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.8
Macrophomina phaseolina
-
tassi; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.8
-
Macrophomina phaseolina
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.8
4°C, 0.05 M Tris-HCL buffer, pH 8.2,36% loss of activity after 2 weeks
Macrophomina phaseolina
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.8
myo-inositol hexakisphosphate + H2O
-
114268
Macrophomina phaseolina
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.8
35
-
-
Macrophomina phaseolina
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.8
45
-
60 min, about 95% loss of activity
Macrophomina phaseolina
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.8
5.6
-
-
Macrophomina phaseolina
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.3.8
5.5
7
10 min, stable
Macrophomina phaseolina
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.8
2,4-dinitrophenol
-
Macrophomina phaseolina
3.1.3.8
arsenate
-
Macrophomina phaseolina
3.1.3.8
arsenite
-
Macrophomina phaseolina
3.1.3.8
azide
-
Macrophomina phaseolina
3.1.3.8
EDTA
-
Macrophomina phaseolina
3.1.3.8
F-
-
Macrophomina phaseolina
3.1.3.8
Hg2+
-
Macrophomina phaseolina
3.1.3.8
iodoacetic acid
-
Macrophomina phaseolina
3.1.3.8
phloridzin
-
Macrophomina phaseolina
3.1.3.8
Tartrate
-
Macrophomina phaseolina
3.1.3.8
Zn2+
-
Macrophomina phaseolina
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.8
0.91
-
myo-inositol hexakisphosphate
-
Macrophomina phaseolina
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.8
Ca2+
activates
Macrophomina phaseolina
3.1.3.8
K+
activates
Macrophomina phaseolina
3.1.3.8
Zn2+
activates
Macrophomina phaseolina
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.3.8
additional information
high resistance to the denaturing effect of organic solvents
Macrophomina phaseolina
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.8
-
Macrophomina phaseolina
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.8
4°C, 0.05 M Tris-HCL buffer, pH 8.2,36% loss of activity after 2 weeks
Macrophomina phaseolina
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.8
myo-inositol hexakisphosphate + H2O
-
114268
Macrophomina phaseolina
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.8
35
-
-
Macrophomina phaseolina
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.8
45
-
60 min, about 95% loss of activity
Macrophomina phaseolina
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.8
5.6
-
-
Macrophomina phaseolina
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.3.8
5.5
7
10 min, stable
Macrophomina phaseolina