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Literature summary extracted from

  • Youssef, K.A.; Ghareib, M.; Nour el Dein, M.M.
    Purification and general properties of extracellular phytase from Aspergillus flaviceps (1987), Zentralbl. Mikrobiol., 142, 397-402.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 2,4-dinitrophenol
-
 Aspergillus flavipes
3.1.3.8 arsenate
-
 Aspergillus flavipes
3.1.3.8 arsenite
-
 Aspergillus flavipes
3.1.3.8 azide
-
 Aspergillus flavipes
3.1.3.8 EDTA
-
 Aspergillus flavipes
3.1.3.8 F-
-
 Aspergillus flavipes
3.1.3.8 Hg2+
-
 Aspergillus flavipes
3.1.3.8 iodoacetic acid
-
 Aspergillus flavipes
3.1.3.8 phloridzin
-
 Aspergillus flavipes
3.1.3.8 Tartrate
-
 Aspergillus flavipes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.63
-
 myo-inositol hexakisphosphate
-
 Aspergillus flavipes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
-
 Aspergillus flavipes
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ca2+ activates Aspergillus flavipes
3.1.3.8 Mn2+ activates Aspergillus flavipes
3.1.3.8 Zn2+ activates Aspergillus flavipes

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus flavipes
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 no modification no carbohydrate and phosphorus Aspergillus flavipes

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Aspergillus flavipes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 additional information
-
-
 Aspergillus flavipes

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 4°C, 0.05 M Tris-HCl, pH 8.2, stable for 8 weeks Aspergillus flavipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus flavipes 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 37
-
-
 Aspergillus flavipes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 30 45 30°C: about 55% of maximal activity, 45°C: about 35% of maximal activity Aspergillus flavipes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 45
-
 20 min, about 50% loss of activity, 60 min, 84.4% loss of activity Aspergillus flavipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5.6
-
-
 Aspergillus flavipes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 4 8 pH 4.0: about 50% of maximal activity, pH 8.0: about 25% of maximal activity Aspergillus flavipes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 5 6.8 37°C, 10 min, stable Aspergillus flavipes