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Literature summary extracted from

  • Jareonkitmongkol, S.; Ohya, M.; Watanabe, R.; Takagi, H.; Nakamori, S.
    Partial purification of phytase from a soil isolate bacterium Klebsiella oxytoca MO-3 (1997), J. Ferment. Bioeng., 83, 393-394.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Cu2+
-
 Klebsiella oxytoca
3.1.3.8 EDTA
-
 Klebsiella oxytoca
3.1.3.8 F- NaF Klebsiella oxytoca
3.1.3.8 Fe2+
-
 Klebsiella oxytoca
3.1.3.8 NEM
-
 Klebsiella oxytoca
3.1.3.8 Zn2+
-
 Klebsiella oxytoca

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Klebsiella oxytoca
-
-
-
3.1.3.8 Klebsiella oxytoca
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Klebsiella oxytoca MO-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 partial Klebsiella oxytoca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 45.3
-
-
 Klebsiella oxytoca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 4-nitrophenyl phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca 4-nitrophenol + phosphate
-
 ?
3.1.3.8 AMP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca adenosine + phosphate
-
 ?
3.1.3.8 AMP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 adenosine + phosphate
-
 ?
3.1.3.8 ATP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca ?
-
 ?
3.1.3.8 ATP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 ?
-
 ?
3.1.3.8 diphosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca 2 phosphate
-
 ?
3.1.3.8 diphosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 2 phosphate
-
 ?
3.1.3.8 Glucose 6-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca Glucose + phosphate
-
 ?
3.1.3.8 Glucose 6-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 Glucose + phosphate
-
 ?
3.1.3.8 glycerol 2-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca glycerol + phosphate
-
 ?
3.1.3.8 glycerol 2-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 glycerol + phosphate
-
 ?
3.1.3.8 inositol tetraphosphate + H2O almost the same activity as with myo-inositol hexakisphosphate Klebsiella oxytoca ?
-
 ?
3.1.3.8 inositol triphosphate + H2O 40% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Klebsiella oxytoca 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60
-
 20 min, stable Klebsiella oxytoca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5 6
-
 Klebsiella oxytoca

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 4 8 stable Klebsiella oxytoca