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Literature summary extracted from

  • Lea, P.J.; Fowden, L.
    Amino acid substrate specificity of asparaginyl-, aspartyl- and glutaminyl-tRNA synthetase isolated from higher plants (1973), Phytochemistry, 12, 1903-1916.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.12 putrescine can replace Mg2+ in activation with 27% efficiency in aminoacylation Vigna radiata var. radiata
6.1.1.12 spermidine can replace Mg2+ in activation with 16% efficiency in aminoacylation Vigna radiata var. radiata
6.1.1.12 spermine can replace Mg2+ in activation with 32% efficiency in aminoacylation Vigna radiata var. radiata
6.1.1.18 putrescine can partially replace Mg2+ in activation, with 32% efficiency Vigna radiata var. radiata
6.1.1.18 spermidine can partially replace Mg2+ in activation, with 12% efficiency Vigna radiata var. radiata
6.1.1.18 spermine can partially replace Mg2+ in activation, with 35% efficiency Vigna radiata var. radiata
6.1.1.22 ethanol slight stimulation at low tRNA concentrations Vigna radiata var. radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.12 2-aminomalonic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
6.1.1.12 Br- 0.7 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 CH3COO- 2.0 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 Cl- 2.2 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 ClO3- 0.1 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 CNS- 0.1 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 Cs+ 1.1 M, 50% inhibition of ATP-diphosphate exchange, 0.16 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
6.1.1.12 erythro-3-hydroxyaspartic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
6.1.1.12 I- 0.1 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 IO3- 0.04 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 K+ 2.3 M, 50% inhibition of ATP-diphosphate exchange, 0.22 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
6.1.1.12 Li+ 0.6 M, 50% inhibition of ATP-diphosphate exchange, 0.11 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
6.1.1.12 Na+ 1.4 M, 50% inhibition of ATP-diphosphate exchange, 0.18 M , 50% inhibition of aminoacylation Vigna radiata var. radiata
6.1.1.12 NH4+ 0.9 M, 50% inhibition of ATP-diphosphate exchange, 0.14 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
6.1.1.12 NO3- 0.7 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 p-chloromercuribenzoate
-
 Vigna radiata var. radiata
6.1.1.12 threo-3-hydroxyaspartic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
6.1.1.12 threo-3-Methylaspartic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
6.1.1.12 tRNA above 0.4 mg/ml Vigna radiata var. radiata
6.1.1.18 4-Methyleneglutamine
-
 Vigna radiata var. radiata
6.1.1.18 albizziine
-
 Vigna radiata var. radiata
6.1.1.18 Br- 0.1 M, 50% inhibition Vigna radiata var. radiata
6.1.1.18 Cl- 0.2 M, 50% inhibition Vigna radiata var. radiata
6.1.1.18 Glutamic acid 4-hydroxamate
-
 Vigna radiata var. radiata
6.1.1.18 I- 0.06 M, 50% inhibition Vigna radiata var. radiata
6.1.1.18 S-Carbamoylcysteine
-
 Vigna radiata var. radiata
6.1.1.18 S-Carbamoylserine
-
 Vigna radiata var. radiata
6.1.1.18 Theanine
-
 Vigna radiata var. radiata
6.1.1.18 tRNA above 0.6 mg/ml Vigna radiata var. radiata
6.1.1.22 3-cyanoalanine aminoacylation of Asn, competitive Vigna radiata var. radiata
6.1.1.22 Aspartate 3-hydroxamate Asn-dependent ATP-diphosphate exchange, competitive Vigna radiata var. radiata
6.1.1.22 Br- 1.2 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 CH3COO- 1.3 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 Cl- 2.5 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 CNS- 0.4 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 Cs+ 1.3 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 I- 0.5 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 isoasparagine aminoacylation of Asn, competitive Vigna radiata var. radiata
6.1.1.22 K+ 2.5 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 Li+ 0.7 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 Na+ 2.0 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 NH4+ 0.9 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 NO3- 1.0 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 p-chloromercuribenzoate
-
 Vigna radiata var. radiata
6.1.1.22 threo-3-Hydroxyasparagine aminoacylation of Asn, competitive Vigna radiata var. radiata
6.1.1.22 Tris above 0.3 M Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.12 4.6
-
 Asp ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 8
-
 2-aminomalonic acid ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 24
-
 threo-3-hydroxyaspartic acid ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 0.12
-
 Asn tRNA esterification Vigna radiata var. radiata
6.1.1.22 0.18
-
 Asn ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 0.26
-
 isoasparagine ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 0.78
-
 aspartate-3-hydroxamate ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.22 5.8
-
 3-cyanoalanine ATP-diphosphate exchange Vigna radiata var. radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.12 Co2+ can replace Mg2+, with 12% efficiency in activation of ATP-diphosphate exchange, with 34% efficiency in aminoacylation Vigna radiata var. radiata
6.1.1.12 Mg2+ required Vigna radiata var. radiata
6.1.1.12 Mg2+ optimal concentration: 12 mM, ATP-diphosphate exchange, 5 mM, aminoacylation Vigna radiata var. radiata
6.1.1.12 Mg2+ Km: 6.9 mM , ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.12 Mg2+ Km: 1.1 mM , aminoacylation Vigna radiata var. radiata
6.1.1.12 Mn2+ can replace Mg2+, with 35% efficiency in activation of ATP-diphosphate exchange, with 56% efficiency in aminoacylation Vigna radiata var. radiata
6.1.1.18 Mg2+ required Vigna radiata var. radiata
6.1.1.18 Mg2+ optimal concentration: 25 mM Vigna radiata var. radiata
6.1.1.18 Mg2+ Km: 8.9 mM Vigna radiata var. radiata
6.1.1.18 Mn2+ can replace Mg2+ in activation with 42% efficiency Vigna radiata var. radiata
6.1.1.22 Ca2+ stimulates tRNA esterification with 5% of the efficiency compared to Mg2+ Vigna radiata var. radiata
6.1.1.22 Co2+ stimulates ATP-diphosphate exchange with 17% the efficiency compared to Mg2+ and tRNA esterification with 45% Vigna radiata var. radiata
6.1.1.22 Mg2+ required, optimal concentration is 20 mM in ATP-diphosphate exchange and 12 mM in tRNA esterification, Km: 5.8 mM in ATP-diphosphate exchange, 2.8 in tRNA esterification Vigna radiata var. radiata
6.1.1.22 Mn2+ stimulates ATP-diphosphate exchange with 30% the efficiency compared to Mg2+ and tRNA esterification with 72% efficiency Vigna radiata var. radiata
6.1.1.22 Ni2+ stimulates tRNA esterification with 15% of the efficiency compared to Mg2+ Vigna radiata var. radiata

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Vigna radiata var. radiata
-
-
-
6.1.1.18 Albizia julibrissin
-
-
-
6.1.1.18 Vigna radiata var. radiata
-
-
-
6.1.1.22 Vicia faba
-
-
-
6.1.1.22 Vicia sativa
-
-
-
6.1.1.22 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12
-
 Vigna radiata var. radiata
6.1.1.18
-
 Vigna radiata var. radiata
6.1.1.22
-
 Vigna radiata var. radiata
6.1.1.22
-
 Vicia faba
6.1.1.22
-
 Vicia sativa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
6.1.1.12 denaturation by 4 M urea, 30% recovery after dialysis Vigna radiata var. radiata
6.1.1.22 concentrations of urea above 4 M inactivate during incubation at 37°C for 5 min. 70% of the activity is recovered by dialysis Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.18 cotyledon of seedlings Albizia julibrissin
-
6.1.1.18 seed
-
 Vigna radiata var. radiata
-
6.1.1.22 seed
-
 Vigna radiata var. radiata
-
6.1.1.22 seed
-
 Vicia faba
-
6.1.1.22 seed
-
 Vicia sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.12 0.326
-
-
 Vigna radiata var. radiata
6.1.1.18 additional information
-
-
 Vigna radiata var. radiata
6.1.1.18 additional information
-
-
 Albizia julibrissin
6.1.1.22 0.287
-
-
 Vicia sativa
6.1.1.22 0.472
-
-
 Vicia faba
6.1.1.22 0.566
-
-
 Vigna radiata var. radiata

Storage Stability

EC Number Storage Stability Organism
6.1.1.12 -20°C, 40% w/v glycerol, SH-group stabilizing reagent, 50% loss of activity after 72 h Vigna radiata var. radiata
6.1.1.12 in a dry form, e.g. as acetone precipitate, stable for 1 month Vigna radiata var. radiata
6.1.1.22 -20°C, 40% w/v glycerol, 2 months with less than 10% loss of activity Vigna radiata var. radiata
6.1.1.22 4°C, 15% w/v glycerol, 48 h, stable Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + 2-aminomalonic acid + tRNAAsp
-
 Vigna radiata var. radiata ?
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Vigna radiata var. radiata AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + threo-3-hydroxyaspartic acid + tRNAAsp
-
 Vigna radiata var. radiata ?
-
 ?
6.1.1.12 additional information enzyme can also utilize 2-aminomalonic acid and threo-3-hydroxyaspartic acid in ATP-diphosphate exchange Vigna radiata var. radiata ?
-
 ?
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Vigna radiata var. radiata AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Albizia julibrissin AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.1.1.18 additional information glutamine-dependent ATP-diphosphate exchange Vigna radiata var. radiata ?
-
 ?
6.1.1.22 ATP + 3-cyanoalanine + tRNAAsn
-
 Vigna radiata var. radiata ?
-
 ?
6.1.1.22 ATP + aspartate-3-hydroxamate + tRNAAsn
-
 Vigna radiata var. radiata ?
-
 ?
6.1.1.22 ATP + isoasparagine + tRNAAsn
-
 Vigna radiata var. radiata ?
-
 ?
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Vigna radiata var. radiata AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Vicia faba AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Vicia sativa AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
6.1.1.22 additional information ATP-diphosphate exchange Vigna radiata var. radiata ?
-
 ?
6.1.1.22 additional information acylate Phaseolus aureus tRNA with an efficiency of 68% compared to the Phaseolus aureus enzyme. The tRNA of Vicia faba and Vicia sativa appears to be completely interchangeable Vicia faba ?
-
 ?
6.1.1.22 additional information acylate Phaseolus aureus tRNA with an efficiency of 68% compared to the Phaseolus aureus enzyme. The tRNA of Vicia faba and Vicia sativa appears to be completely interchangeable Vicia sativa ?
-
 ?
6.1.1.22 additional information acylates tRNA of Vicia faba or Vicia sativa with an efficiency of 83% compared to Phaseolus aureus tRNA Vigna radiata var. radiata ?
-
 ?
6.1.1.22 additional information isoasparagine (with 96% of the efficiency compared to L-Asn), aspartate-3-hydroxamate (with 81% of the efficiency compared to L-Asn) and 3-cyanoalanine (with 36% of the efficiency compared to L-Asn) Vigna radiata var. radiata ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.12 50
-
 half-life: 7 min without stabilizing reagents, 12 min in presence of 0.004 mM ATP, 23 min in presence of 0.004 mM ATP and 0.1 mM Asp Vigna radiata var. radiata
6.1.1.22 50
-
 50% loss of activity after 5 min (without addition of stabilizer), after 14 min (with addition of 0.004 mM ATP), after 27 min (with addition of 0.05 mM Asn and ATP) Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.12 8
-
 ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.18 7.8
-
-
 Vigna radiata var. radiata
6.1.1.22 7.8
-
 ATP-diphosphate exchange, tRNA esterification, Tris-chloride or Tris-acetate buffer Vigna radiata var. radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.12 7.8 9.1 7.8: sharp loss of activity below, 9.1: plateau of high activity up to pH 9.1 Vigna radiata var. radiata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.12 6000
-
 Li+ ATP-diphosphate exchange Vigna radiata var. radiata
6.1.1.18 0.06
-
 I-
-
 Vigna radiata var. radiata
6.1.1.18 0.1
-
 Br-
-
 Vigna radiata var. radiata
6.1.1.18 0.2
-
 Cl-
-
 Vigna radiata var. radiata