EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.2 | Gly | stimulates | Rattus norvegicus | |
6.3.2.2 | Gly | no effect | Xenopus laevis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.2 | glutathione | inhibited by both GSSG and GSH | Rattus norvegicus | |
6.3.2.2 | glutathione | GSSG inhibits, GSH has no inhibitory effect | Xenopus laevis | |
6.3.2.2 | K+ | - |
Rattus norvegicus | |
6.3.2.2 | K+ | - |
Xenopus laevis | |
6.3.2.2 | NAD+ | - |
Rattus norvegicus | |
6.3.2.2 | NAD+ | - |
Xenopus laevis | |
6.3.2.2 | NADH | - |
Rattus norvegicus | |
6.3.2.2 | NADH | - |
Xenopus laevis | |
6.3.2.2 | NADP+ | - |
Rattus norvegicus | |
6.3.2.2 | NADP+ | - |
Xenopus laevis | |
6.3.2.2 | NADPH | - |
Rattus norvegicus | |
6.3.2.2 | NADPH | - |
Xenopus laevis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.2 | Rattus norvegicus | - |
- |
- |
6.3.2.2 | Xenopus laevis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.2 | - |
Rattus norvegicus |
6.3.2.2 | - |
Xenopus laevis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine | bi bi uni uni ping pong mechanism, with glutamyl-enzyme as intermediate before the addition of cys and the release of gamma-glutamylcysteine | Xenopus laevis | |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine | tri tri sequential mechanism | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.2.2 | liver | - |
Rattus norvegicus | - |
6.3.2.2 | liver | - |
Xenopus laevis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.2.2 | additional information | - |
- |
Rattus norvegicus |
6.3.2.2 | additional information | - |
- |
Xenopus laevis |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.2.2 | 4°C, or in the frozen state, 25-30% loss of activity | Xenopus laevis |
6.3.2.2 | 4°C, or in the frozen state, stable for 2 weeks | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.2 | ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
6.3.2.2 | ATP + L-Glu + L-Cys | - |
Xenopus laevis | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.2 | 7.7 | - |
- |
Rattus norvegicus |
6.3.2.2 | 7.7 | - |
- |
Xenopus laevis |