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Literature summary extracted from

  • Seelig, G.F.; Meister, A.
    gamma-Glutamylcysteine synthetase from erythrocytes (1984), Anal. Biochem., 141, 510-514.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 cystamine 7.5 mM MgCl2 + 7.5 mM L-Glu protect Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 0.35
-
 ATP
-
 Rattus norvegicus
6.3.2.2 1.2
-
 L-Glu
-
 Rattus norvegicus
6.3.2.2 1.5
-
 L-2-aminobutanoate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 25000
-
 1 * 75000 + 1 * 25000, denaturing PAGE in presence of 50 mM DTT Rattus norvegicus
6.3.2.2 75000
-
 1 * 75000 + 1 * 25000, denaturing PAGE in presence of 50 mM DTT Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 erythrocyte
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 24.3
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-2-aminobutanoate
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 dimer 1 * 75000 + 1 * 25000, denaturing PAGE in presence of 50 mM DTT Rattus norvegicus