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Literature summary extracted from

  • Garrow, T.A.; Shane, B.
    Purification and general properties of human folylpolyglutamate synthetase (1993), Chem. Biol. Pteridines Folates (J. E. Ayling et al eds. ) Plenum Press New York, , 659-662.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.17 Reducing agents absolutely dependent on Homo sapiens
6.3.2.17 Reducing agents beta-mercaptoethanol or DTT Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.17 expression in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.0009
-
 7,8-dihydropteroyl-Glu
-
 Homo sapiens
6.3.2.17 0.002
-
 5,6,7,8-tetrahydropteroyl-Glu4 5,6,7,8-tetrahydropteroyl-Glu Homo sapiens
6.3.2.17 0.0037
-
 10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
 Homo sapiens
6.3.2.17 0.0044
-
 5,6,7,8-tetrahydropteroyl-Glu aminopterin Homo sapiens
6.3.2.17 0.048
-
 7,8-dihydropteroyl-Glu2
-
 Homo sapiens
6.3.2.17 0.06
-
 pteroyl-Glu
-
 Homo sapiens
6.3.2.17 0.081
-
 methotrexate
-
 Homo sapiens
6.3.2.17 0.105
-
 5-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 K+ absolutely dependent on monovalent cation, K+ is most effective Homo sapiens
6.3.2.17 Mn2+ stimulates Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 60000
-
 x * 60000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
 Homo sapiens ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Homo sapiens ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Homo sapiens ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
 Homo sapiens ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Homo sapiens ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glu + L-glutamate
-
 Homo sapiens ADP + phosphate + 7,8-dihydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Homo sapiens ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
6.3.2.17 ATP + aminopterin + Glu
-
 Homo sapiens ADP + phosphate + aminopteryl-Glu
-
 ?
6.3.2.17 ATP + methotrexate + Glu
-
 Homo sapiens ADP + phosphate + methotrexyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Homo sapiens ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.17 ? x * 60000, SDS-PAGE Homo sapiens