Literature summary extracted from
Wiegert, T.; Sahm, H.; Sprenger, G.A.
The substitution of a single amino acid residue (Ser-116->Asp) alters NADP-containing glucose-fructose oxidoreductase of Zymomonas mobilis into a glucose dehydrogenase with dual coenzyme specificity (1997), J. Biol. Chem., 272, 13126-13133.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.1.99.28 |
S116D |
a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type |
Zymomonas mobilis |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.1.99.28 |
additional information |
- |
additional information |
kinetic parameters of the glucose dehydrogenase reaction catalyzed by mutant enzymes |
Zymomonas mobilis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.99.28 |
Zymomonas mobilis |
Q07982 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.99.28 |
mutant enzyme S116D |
Zymomonas mobilis |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.1.99.28 |
additional information |
- |
kinetic parameters of the glucose dehydrogenase reaction catalyzed by mutant enzymes |
Zymomonas mobilis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.99.28 |
D-glucose + D-fructose |
- |
Zymomonas mobilis |
D-glucono-1,5-lactone + D-sorbitol |
- |
? |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.99.28 |
NAD+ |
a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type |
Zymomonas mobilis |
|
1.1.99.28 |
NADH |
a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type |
Zymomonas mobilis |
|
1.1.99.28 |
NADP+ |
a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type |
Zymomonas mobilis |
|
1.1.99.28 |
NADPH |
a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type |
Zymomonas mobilis |
|