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Literature summary extracted from

  • Imeson, H.C.; Cossins, E.A.
    Folylpolyglutamate synthase from higher plants (1997), Methods Enzymol., 281, 141-145.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.17 50% glycerol stabilizes for several days at 4°C Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.0035
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Pisum sativum
6.3.2.17 0.61
-
 L-Glu
-
 Pisum sativum
6.3.2.17 2.3
-
 ATP
-
 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.17 cytosol
-
 Homo sapiens 5829
-
6.3.2.17 cytosol cotyledon Pisum sativum 5829
-
6.3.2.17 mitochondrion leaf Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 NaHCO3 optimal activation at 10 mM Homo sapiens
6.3.2.17 NaHCO3 optimal activation at 10 mM Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Homo sapiens
-
-
-
6.3.2.17 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.17 cotyledon
-
 Pisum sativum
-
6.3.2.17 leaf
-
 Pisum sativum
-
6.3.2.17 seed
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.17 additional information
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
6.3.2.17 purified enzyme loses activity within 1-2 days at 4, without stabilizer Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Pisum sativum ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
 Pisum sativum ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
-
 Homo sapiens ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
-
 Pisum sativum ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 8.5
-
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.17 7 10 7.4: about 50% of maximal activity Pisum sativum