EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.12 | additional information | - |
additional information | Km value of pure tRNA, transcript tRNA and unfractionated tRNAs of various origins with enzyme form AspRS1 and enzyme form AspRS2 | Thermus thermophilus | |
6.1.1.12 | 0.003 | - |
Asp | 37°C and 70°C, tRNA aminoacylation, enzyme form AspRS2 | Thermus thermophilus | |
6.1.1.12 | 0.009 | - |
Asp | 37°C, tRNA aminoacylation, enzyme form AspRS1 | Thermus thermophilus | |
6.1.1.12 | 0.03 | - |
Asp | 37°C, ATP-diphosphate exchange, and 70°C, tRNAaminoacylation, enzyme form AspRS1 | Thermus thermophilus | |
6.1.1.12 | 0.062 | - |
Asp | 37°C, ATP-diphosphate exchange, enzyme form AspRS2 | Thermus thermophilus | |
6.1.1.12 | 0.1 | - |
Asp | 70°C, ATP-diphosphate exchange by enzyme form AspRS1, and tRNA aminoacylation by enzyme form AspRS2 | Thermus thermophilus | |
6.1.1.12 | 0.12 | - |
ATP | 37°C, tRNA aminoacylation, enzyme form AspRS1 | Thermus thermophilus | |
6.1.1.12 | 0.25 | - |
ATP | 70°C, ATP-diphosphate exchange, enzyme form AspRS1 | Thermus thermophilus | |
6.1.1.12 | 0.28 | - |
ATP | 70°C, tRNA aminoacylation, enzyme form AspRS1 | Thermus thermophilus | |
6.1.1.12 | 0.37 | - |
ATP | 37°C, ATP-diphosphate exchange, enzyme form AspRS1 | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.12 | Thermus thermophilus | - |
- |
- |
6.1.1.12 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.12 | 2 distinct enzyme forms: AspRS1 and AspRS2 | Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.1.1.12 | 0.0405 | - |
enzyme form AspRS2 | Thermus thermophilus |
6.1.1.12 | 0.0452 | - |
enzyme form AspRS1 | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.12 | ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | additional information | aspartate-dependent ATP-diphosphate exchange | Thermus thermophilus | ? | - |
? | |
6.1.1.12 | additional information | aspartate-dependent ATP-diphosphate exchange | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? |