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Literature summary extracted from

  • Cichowicz, D.J.; Foo, S.K.; Shane, B.
    Folylpoly-gamma-glutamate synthesis by bacteria and mammalian cells (1981), Mol. Cell. Biochem., 39, 209-228.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.17 Borate
-
 Corynebacterium sp.
6.3.2.17 phosphate
-
 Corynebacterium sp.
6.3.2.17 phosphate
-
 Lacticaseibacillus casei
6.3.2.17 phosphate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 K+ required form maximal activity Escherichia coli
6.3.2.17 K+ required form maximal activity Sus scrofa
6.3.2.17 K+ required form maximal activity Corynebacterium sp.
6.3.2.17 K+ required form maximal activity Lacticaseibacillus casei
6.3.2.17 K+ maximal activity at 200 mM Escherichia coli
6.3.2.17 K+ maximal activity at 200 mM Sus scrofa
6.3.2.17 K+ maximal activity at 200 mM Corynebacterium sp.
6.3.2.17 K+ maximal activity at 200 mM Lacticaseibacillus casei
6.3.2.17 Mg2+ required Escherichia coli
6.3.2.17 Mg2+ required Sus scrofa
6.3.2.17 Mg2+ required Corynebacterium sp.
6.3.2.17 Mg2+ required Lacticaseibacillus casei
6.3.2.17 Mg2+ optimal Mg2+ concentration is dependent on ATP concentration Corynebacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 51000
-
 gel filtration Corynebacterium sp.
6.3.2.17 53000
-
 1 * 53000, SDS-PAGE Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Corynebacterium sp.
-
-
-
6.3.2.17 Cricetulus griseus
-
-
-
6.3.2.17 Enterococcus faecalis
-
-
-
6.3.2.17 Escherichia coli
-
-
-
6.3.2.17 Lacticaseibacillus casei
-
-
-
6.3.2.17 Neurospora crassa
-
-
-
6.3.2.17 Rattus norvegicus
-
-
-
6.3.2.17 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17
-
 Corynebacterium sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.17 ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate = ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1 formation of a pteroyl-gamma-glutamyl phosphate intermediate Corynebacterium sp.
a
6.3.2.17 ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate = ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1 ordered ter ter mechanism with MgATP binding first to the enzyme, folate second, and glutamate last Corynebacterium sp.
a
6.3.2.17 ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate = ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1 ordered ter ter mechanism with MgATP binding first to the enzyme, folate second, and glutamate last Lacticaseibacillus casei
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.17 liver
-
 Rattus norvegicus
-
6.3.2.17 liver
-
 Sus scrofa
-
6.3.2.17 ovary
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.17 0.57
-
-
 Corynebacterium sp.

Storage Stability

EC Number Storage Stability Organism
6.3.2.17 -20°C or -196°C, 50 mM potassium phosphate buffer, pH 7, 50 mM KCl, 30% DMSO Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Escherichia coli ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Rattus norvegicus ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Sus scrofa ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Rattus norvegicus ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Neurospora crassa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroate + Glu
-
 Corynebacterium sp. ADP + phosphate + 5,6,7,8-terahydropteroyl-Glu
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Neurospora crassa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Rattus norvegicus ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Sus scrofa ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Neurospora crassa ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Lacticaseibacillus casei ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Rattus norvegicus ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Lacticaseibacillus casei ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Neurospora crassa ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroate + Glu
-
 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glu
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroate + Glu
-
 Corynebacterium sp. ADP + phosphate + 7,8-dihydropteroyl-Glu
-
 ?
6.3.2.17 ATP + aminopterin + Glu
-
 Rattus norvegicus ADP + phosphate + aminopteryl-Glu
-
 ?
6.3.2.17 ATP + aminopterin + Glu
-
 Sus scrofa ADP + phosphate + aminopteryl-Glu
-
 ?
6.3.2.17 ATP + aminopterin + Glu
-
 Corynebacterium sp. ADP + phosphate + aminopteryl-Glu
-
 ?
6.3.2.17 ATP + aminopterin + Glu
-
 Lacticaseibacillus casei ADP + phosphate + aminopteryl-Glu
-
 ?
6.3.2.17 additional information
-
 Escherichia coli ?
-
 ?
6.3.2.17 additional information
-
 Neurospora crassa ?
-
 ?
6.3.2.17 additional information
-
 Lacticaseibacillus casei ?
-
 ?
6.3.2.17 additional information dATP and UTP can act as substrate Corynebacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.17 monomer
-
 Escherichia coli
6.3.2.17 monomer
-
 Sus scrofa
6.3.2.17 monomer
-
 Lacticaseibacillus casei
6.3.2.17 monomer 1 * 53000, SDS-PAGE Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 9.5 10
-
 Corynebacterium sp.