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Literature summary extracted from

  • Cichowicz, D.J.; Shane, B.
    Mammalian folylpoly-gamma-glutamate synthetase. 2. Substrate specificity and kinetic properties (1987), Biochemistry, 26, 513-521.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.17 4-threo-Fluoroglutamate prevents or severly retards further addition of Glu Sus scrofa
6.3.2.17 adenosine 5'-O-(3-thiotriphosphate)
-
 Sus scrofa
6.3.2.17 ADP
-
 Sus scrofa
6.3.2.17 aminopterin
-
 Sus scrofa
6.3.2.17 AMP
-
 Sus scrofa
6.3.2.17 ATP4-
-
 Sus scrofa
6.3.2.17 beta,gamma-Imido-ATP
-
 Sus scrofa
6.3.2.17 beta,gamma-methylene-ATP
-
 Sus scrofa
6.3.2.17 diphosphate weak, competitive with respect to MgATP2- Sus scrofa
6.3.2.17 L-Homocysteate prevents or severly retards further addition of Glu Sus scrofa
6.3.2.17 P1,P5-di(adenosine-5')pentaphosphate
-
 Sus scrofa
6.3.2.17 phosphate competitive with respect to MgATP2- Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.0011
-
 5,6,7,8-tetrahydropteroyl-Glu3 5,6,7,8-tetrahydropteroyl-Glu5 Sus scrofa
6.3.2.17 0.002
-
 5,6,7,8-tetrahydropteroyl-Glu4
-
 Sus scrofa
6.3.2.17 0.0022
-
 10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
6.3.2.17 0.0026
-
 7,8-dihydropteroyl-Glu2
-
 Sus scrofa
6.3.2.17 0.0027
-
 5,6,7,8-tetrahydropteroyl-Glu5
-
 Sus scrofa
6.3.2.17 0.0034
-
 5,6,7,8-tetrahydropteroyl-Glu2
-
 Sus scrofa
6.3.2.17 0.005
-
 7,8-dihydropteroyl-Glu
-
 Sus scrofa
6.3.2.17 0.006
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
6.3.2.17 0.0077
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
6.3.2.17 0.01
-
 ATP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
6.3.2.17 0.017
-
 aminopterin
-
 Sus scrofa
6.3.2.17 0.05
-
 dATP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
6.3.2.17 0.054
-
 methotrexate 5-methyl-5,6,7,8-tetrahydrpteroyl-Glu Sus scrofa
6.3.2.17 0.057
-
 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
6.3.2.17 0.062
-
 pteroylmonoglutamyl-Glu pteroyl-Glu2 Sus scrofa
6.3.2.17 0.115
-
 pteroyl-Glu
-
 Sus scrofa
6.3.2.17 0.119
-
 pteroyl-Glu3
-
 Sus scrofa
6.3.2.17 0.136
-
 ITP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
6.3.2.17 0.19
-
 UTP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
6.3.2.17 0.336
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
6.3.2.17 0.346
-
 Glu reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
6.3.2.17 0.962
-
 pteroylglutamate
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Sus scrofa
-
 hog
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.17 ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate = ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1 ordered ter-ter mechanism with MgATP binding first, folate second and Glu last Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.17 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Sus scrofa ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Sus scrofa ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-Glu
-
 Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
-
 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu4
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
-
 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu5
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
-
 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu6
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 3 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 5 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 7 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Sus scrofa ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroate + Glu
-
 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glu
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 5 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
6.3.2.17 ATP + aminopterin + Glu
-
 Sus scrofa ADP + phosphate + aminopteryl-Glu
-
 ?
6.3.2.17 ATP + methotrexate + Glu
-
 Sus scrofa ADP + phosphate + methotrexyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 3 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 5 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 2 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 4 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 7 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 6 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
6.3.2.17 ATP + pteroyl-Glu3 + L-glutamate
-
 Sus scrofa ADP + phosphate + pteroyl-Glu4
-
 ?
6.3.2.17 dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Sus scrofa dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ITP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Sus scrofa IDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Sus scrofa UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.17 1.7
-
 L-Glu
-
 Sus scrofa
6.3.2.17 2
-
 aminopterin
-
 Sus scrofa